Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SAP130 antibody at 1:500 dilution.)
Rabbit SAP130 Polyclonal Antibody | anti-SAP130 antibody
SAP130 Antibody
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
SAP130; Polyclonal Antibody; SAP130 Antibody; Affinity Purified Rabbit Polyclonal SAP130 Antibody; Polyclonal SAP130 antibody; Anti-SAP130 antibody; SF3b130 antibody; pre-mRNA splicing factor SF3b 130 kDa subunit antibody; KIAA0017 antibody; pre-mRNA-splicing factor SF3b 130 kDa subunit antibody; RSE1 antibody; splicing factor 3B subunit 3 antibody; SAP 130; SAP-130; SAP 130 antibody; SAP-130 antibody; SAP130 antibody; SF3B3 antibody; STAF130 antibody; anti-SAP130 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
669
Applicable Applications for anti-SAP130 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:250
WB: 1:500-1:3000
WB: 1:500-1:3000
Immunogen
SAP130 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 1154 and 1217 of SAP130
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SAP130 antibody at 1:500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of methanol-fixed HeLa, using SAP130 antibody at 1:500 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 5% SDS PAGE gel and SAP130 antibody at a dilution of 1:3000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 5% SDS PAGE gel and SAP130 antibody at a dilution of 1:3000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Saos-2 xenograft using SAP130 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Saos-2 xenograft using SAP130 antibody at a dilution of 1:100)
Related Product Information for anti-SAP130 antibody
This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex. The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA 31-GCN5L acetylase) transcription coactivator-HAT complex, and the TFTC-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.
Product Categories/Family for anti-SAP130 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
136 kDa
NCBI Official Full Name
SAP130, partial
Protein Family
Similar Products
Product Notes
The SAP130 (Catalog #AAA5312095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SAP130 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:250 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the SAP130 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP130, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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