Rabbit SAFB Polyclonal Antibody | anti-SAFB antibody

SAFB Antibody

Cat. Num. AAA9612205

• Gene Names: SAFB; HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SAFB; Polyclonal Antibody; SAFB Antibody; DKFZp779C1727; glutathione S transferase fusion protein; HAP; HET; HSP27 ERE TATA binding protein; HSP27 ERE-TATA-binding protein; HSP27 estrogen response element-TATA box-binding protein; SAF B; SAF-B; SAF-B1; SAFB 1; SAFB1; SAFB1_HUMAN; Scaffold attachment factor B1; anti-SAFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SAFB Antibody detects endogenous levels of total SAFB.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 914

• Applicable Applications for anti-SAFB antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SAFB, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using SAFB Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-SAFB antibody

Function: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.

Post Translational Modifications: Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.

Subcellular Location: Nucleus.

Tissue Specificity: Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Subunit Structure: Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity. Interacts with RBMX (By similarity). Interacts with FUS.

NCBI and Uniprot Product Information

• NCBI GI #: 1002095852
• NCBI GeneID: 6294
• NCBI Accession #: NP_001307501.1
• NCBI GenBank Nucleotide #: NP_001307501.1
• UniProt Accession #: Q15424
• Molecular Weight: Observed Molecular Weight: (Observed)102kD.; Predicted Molecular Weight: (Calculated)103kDa.
• NCBI Official Full Name: scaffold attachment factor B1 isoform 6
• NCBI Official Synonym Full Names: scaffold attachment factor B
• NCBI Official Symbol: SAFB
• NCBI Official Synonym Symbols: HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1
• NCBI Protein Information: scaffold attachment factor B1
• UniProt Protein Name: Scaffold attachment factor B1
• Protein Family: Scaffold attachment factor
• UniProt Gene Name: SAFB
• UniProt Synonym Gene Names: HAP; HET; SAFB1; SAF-B; SAF-B1; HSP27 ERE-TATA-binding protein

NCBI Description

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (). Can inhibit cell proliferation.