Rabbit S6 Ribosomal Protein Polyclonal Antibody | anti-RPS6 antibody

S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody

Cat. Num. AAA9508665

• Gene Names: RPS6; S6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: S6 Ribosomal Protein; Polyclonal Antibody; S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody; 40S ribosomal protein S6; Phosphoprotein NP33; ribosomal protein S6; RPS6; RS6; anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody detects endogenous levels of RPS6 only when phosphorylated at Ser235+Ser236.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 249

• Applicable Applications for anti-RPS6 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S235+S236; M:S235+S236; R:S235+S236
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human S6 Ribosomal Protein around the phosphorylation site of Ser235+Ser236.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody)

Western Blot (WB)

(Western blot analysis of Jurkat treated with TNF and NIH/3T3 cells treated with PMA, using S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 17158044
• NCBI GeneID: 6194
• NCBI Accession #: NP_001001.2
• NCBI GenBank Nucleotide #: NM_001010.2
• UniProt Accession #: P62753; P08227; P10660; Q4VBY7; Q8N6Z7
• Molecular Weight: 28 kDa
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: RPS6
• NCBI Official Synonym Symbols: S6
• NCBI Protein Information: 40S ribosomal protein S6
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS6

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.