Human Nuclear Factor I/B (NFIB) ELISA Kit | NFIB elisa kit

Human Nuclear Factor I/B (NFIB) ELISA Kit

Cat. Num. AAA454749

• Gene Names: NFIB; CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• Reactivity: Human
• Synonyms: Nuclear Factor I/B (NFIB); Human Nuclear Factor I/B (NFIB) ELISA Kit; NFI-RED; NFIB2; NFIB3; CCAAT-box-binding transcription factor; TGGCA-binding protein; NFIB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NFIB. No significant cross-reactivity or interference between NFIB and analogues was observed.
• Sequence Length: 494

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFIB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFIB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFIB elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NFIB in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFIB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NFIB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NFIB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 299758476
• NCBI GeneID: 4781
• NCBI Accession #: NP_001177666.1
• NCBI GenBank Nucleotide #: NM_001190737.1
• UniProt Accession #: O00712; O00166; Q12858; Q5VW29; Q63HM5; Q6ZNF9; Q96J45; G3V1P1; H7BYE8
• Molecular Weight: 33,525 Da
• NCBI Official Full Name: nuclear factor 1 B-type isoform 1
• NCBI Official Synonym Full Names: nuclear factor I B
• NCBI Official Symbol: NFIB
• NCBI Official Synonym Symbols: CTF; NF1-B; NFI-B; NFIB2; NFIB3; NF-I/B; NFI-RED; HMGIC/NFIB
• NCBI Protein Information: nuclear factor 1 B-type
• UniProt Protein Name: Nuclear factor 1 B-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIB
• UniProt Synonym Gene Names: NF1-B; Nuclear factor 1/B; CTF; NF-I/B; NFI-B

Uniprot Description

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.