Rabbit anti-Human, Mouse S100Z Polyclonal Antibody | anti-S100Z antibody

S100Z, NT (S100Z, Protein S100-Z, S100 calcium-binding protein Z) (Biotin)

Cat. Num. AAA6344680

• Gene Names: S100Z; Gm625; S100-zeta
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: S100Z; Polyclonal Antibody; NT (S100Z; Protein S100-Z; S100 calcium-binding protein Z) (Biotin); S100 calcium-binding protein Z; anti-S100Z antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-S100Z antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: S100Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human S100Z.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(S100Z Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(S100Z Antibody (N-term) western blot analysis in MDA-MB453,K562,SK-BR-3,HepG2 cell line and mouse stomach tissue lysates (35ug/lane).This demonstrates the S100Z antibody detected the S100Z protein (arrow).)

Related Product Information for anti-S100Z antibody

Members of the S100 protein family contain 2 calcium-binding EF-hands and exhibit cell-type specific expression patterns. For additional background information on S100 proteins, see MIM 114085.

Product Categories/Family for anti-S100Z antibody

Antibodies; Binding Proteins; Calcium

References

Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010). Gribenko, A.V., et al. Biochemistry 40(51):15538-15548(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 150010678
• NCBI GeneID: 170591
• NCBI Accession #: NP_570128.2
• NCBI GenBank Nucleotide #: NM_130772.3
• UniProt Accession #: Q8WXG8
• Molecular Weight: 11,620 Da
• NCBI Official Full Name: protein S100-Z
• NCBI Official Synonym Full Names: S100 calcium binding protein Z
• NCBI Official Symbol: S100Z
• NCBI Official Synonym Symbols: Gm625; S100-zeta
• NCBI Protein Information: protein S100-Z; S100 calcium-binding protein Z; S100 calcium binding protein, zeta
• UniProt Protein Name: Protein S100-Z
• Protein Family: Protein
• UniProt Gene Name: S100Z
• UniProt Entry Name: S100Z_HUMAN

NCBI Description

Members of the S100 protein family contain 2 calcium-binding EF-hands and exhibit cell-type specific expression patterns. For additional background information on S100 proteins, see MIM 114085.[supplied by OMIM, Mar 2008]

Uniprot Description

S100Z: Belongs to the S-100 family. Homodimer. Interacts with S100P

Chromosomal Location of Human Ortholog: 5q13.3

Molecular Function: calcium ion binding