Rabbit anti-Human S100 Calcium Binding Protein A12 Polyclonal Antibody | anti-S100A12 antibody

S100 Calcium Binding Protein A12 (S100A12, p6, CAGC, CGRP, MRP6, CAAF1, MRP-6, ENRAGE) (HRP)

Cat. Num. AAA6436565

• Gene Names: S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: S100 Calcium Binding Protein A12; Polyclonal Antibody; S100 Calcium Binding Protein A12 (S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE) (HRP); anti-S100A12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human S100A12.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-S100A12 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human S100A12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using S100A12 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human thyroid cancer using S100A12 antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using S100A12 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-S100A12 antibody

Antibodies; Binding Proteins; Calcium

NCBI and Uniprot Product Information

• NCBI GI #: 2507565
• NCBI GeneID: 6283
• UniProt Accession #: P80511; P83219; Q5SY66; Q7M4R1
• Molecular Weight: 92
• NCBI Official Full Name: Protein S100-A12
• NCBI Official Synonym Full Names: S100 calcium binding protein A12
• NCBI Official Symbol: S100A12
• NCBI Official Synonym Symbols: p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• NCBI Protein Information: protein S100-A12; EN-RAGE; calcitermin; calgranulin C; calgranulin-C; neutrophil S100 protein; calcium-binding protein in amniotic fluid 1; migration inhibitory factor-related protein 6; S100 calcium-binding protein A12 (calgranulin C); extracellular newl
• UniProt Protein Name: Protein S100-A12
• Protein Family: Protein
• UniProt Gene Name: S100A12
• UniProt Synonym Gene Names: CAAF1; CAGC; EN-RAGE; MRP-6; p6
• UniProt Entry Name: S10AC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytoplasm; extracellular region; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding

Biological Process: neutrophil chemotaxis; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; mast cell activation; cytokine secretion; activation of NF-kappaB transcription factor; positive regulation of inflammatory response