Rabbit anti-Human S-Phase Kinase Associated Protein 1 (SKP1) Polyclonal Antibody | anti-SKP1 antibody

Cy3-Linked Polyclonal Antibody to S-Phase Kinase Associated Protein 1 (SKP1)

Cat. Num. AAA2077379

• Gene Names: SKP1; ARABIDOPSIS SKP1 HOMOLOGUE 1; ASK1; ATSKP1; S phase kinase-associated protein 1; SKP1A; T4O12.17; T4O12_17; UFO INTERACTING PROTEIN 1; UIP1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Synonyms: S-Phase Kinase Associated Protein 1 (SKP1); Polyclonal Antibody; Cy3-Linked Polyclonal Antibody to S-Phase Kinase Associated Protein 1 (SKP1); SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like; anti-SKP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Concentration: >=100ug/ml (varies by lot)

• Applicable Applications for anti-SKP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (ELISA)
• Immunogen: SKP1 (Met1~Lys163)
• Conjugation: Cy3
• Cross Reactivity: Human, Rat, Pig
• Unconjugated Antibody: The unconjugated antibody version of this item is also available as catalog #MBS2027888

Knockout Validation

(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: SKP1 knockout Hela cell lysate; Predicted MW: 18,19kd Observed MW: 20kd Primary Ab: 2ug/ml Rabbit Anti-Human SKP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 18410982
• NCBI GeneID: 843928
• NCBI Accession #: NP_565123.1
• NCBI GenBank Nucleotide #: NM_106245.5
• UniProt Accession #: Q39255; P92992
• Molecular Weight: 17,857 Da
• NCBI Official Full Name: S phase kinase-associated protein 1
• NCBI Official Symbol: SKP1
• NCBI Official Synonym Symbols: ARABIDOPSIS SKP1 HOMOLOGUE 1; ASK1; ATSKP1; S phase kinase-associated protein 1; SKP1A; T4O12.17; T4O12_17; UFO INTERACTING PROTEIN 1; UIP1
• NCBI Protein Information: S phase kinase-associated protein 1
• UniProt Protein Name: SKP1-like protein 1A
• Protein Family: S-phase kinase-associated protein
• UniProt Gene Name: SKP1A
• UniProt Synonym Gene Names: ASK1; SKP1; UIP1; SKP1-like 1

NCBI Description

SKP1 is core component of the SCF family of E3 ubiquitin ligases and serves to tether the rest of the complex to an F-box protein, which provides specificity in binding to ubiquitin ligase substrate proteins. Predominately expressed from leptotene to pachytene. Negatively regulates recombination. Interacts with P0, a silencing suppressor protein encoded by poleroviruses by means of a conserved minimal F-box motif.

Uniprot Description

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends of the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.