Human S-Phase Kinase Associated Protein 1 (SKP1) ELISA Kit | SKP1 elisa kit

Human S-Phase Kinase Associated Protein 1 (SKP1) ELISA Kit

Cat. Num. AAA2705955

• Gene Names: SKP1; ARABIDOPSIS SKP1 HOMOLOGUE 1; ASK1; ATSKP1; S phase kinase-associated protein 1; SKP1A; T4O12.17; T4O12_17; UFO INTERACTING PROTEIN 1; UIP1
• Reactivity: Human
• Synonyms: S-Phase Kinase Associated Protein 1 (SKP1); Human S-Phase Kinase Associated Protein 1 (SKP1) ELISA Kit; SKP1A; EMC19; OCP-II; OCP2; TCEB1L; p19A; Cyclin-A/CDK2-associated p19; RNA polymerase II elongation factor-like protein; Transcription elongation factor B polypeptide 1-like; SKP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S-Phase Kinase Associated Protein 1 (SKP1).; No significant cross-reactivity or interference between S-Phase Kinase Associated Protein 1 (SKP1) and analogues was observed.
• Sequence Length: 160

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SKP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 1 (SKP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Phase Kinase Associated Protein 1 (SKP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18410982
• NCBI GeneID: 843928
• NCBI Accession #: NP_565123.1
• NCBI GenBank Nucleotide #: NM_106245.5
• UniProt Accession #: Q39255; P92992
• Molecular Weight: 17,857 Da
• NCBI Official Full Name: S phase kinase-associated protein 1
• NCBI Official Symbol: SKP1
• NCBI Official Synonym Symbols: ARABIDOPSIS SKP1 HOMOLOGUE 1; ASK1; ATSKP1; S phase kinase-associated protein 1; SKP1A; T4O12.17; T4O12_17; UFO INTERACTING PROTEIN 1; UIP1
• NCBI Protein Information: S phase kinase-associated protein 1
• UniProt Protein Name: SKP1-like protein 1A
• Protein Family: S-phase kinase-associated protein
• UniProt Gene Name: SKP1A
• UniProt Synonym Gene Names: SKP1-like 1

NCBI Description

SKP1 is core component of the SCF family of E3 ubiquitin ligases and serves to tether the rest of the complex to an F-box protein, which provides specificity in binding to ubiquitin ligase substrate proteins. Predominately expressed from leptotene to pachytene. Negatively regulates recombination. Interacts with P0, a silencing suppressor protein encoded by poleroviruses by means of a conserved minimal F-box motif.

Uniprot Description

Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1. SCF(UFO) is required for vegetative and floral organ development as well as for male gametogenesis. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1), SCF(ADO2), SCF(ADO3) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling. Plays a role during embryogenesis and early postembryonic development, especially during cell elongation and division. Contributes to the correct chromosome segregation during tetrad formation.