Rabbit RYBP Polyclonal Antibody | anti-RYBP antibody

Rabbit Polyclonal to Human RYBP

Cat. Num. AAA240377

• Gene Names: RYBP; AAP1; DEDAF; YEAF1
• Reactivity: Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: RYBP; Polyclonal Antibody; Rabbit Polyclonal to Human RYBP; Anti-RYBP Antibody (aa215-228) IHC-plus; AAP1; APAP-1; Apoptin-associating protein 1; DED-associated factor; DEDAF; Ring1 interactor RYBP; RING1 and YY1 binding protein; YEAF1; RING1 and YY1-binding protein; Human RYBP; anti-RYBP antibody

• Host: Rabbit
• Reactivity: Gibbon, Bovine, Chicken, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: synthetic peptide (TPKGDMSAVNDESF) corresponding to amino acids 215 to 228 of human DEDAF The sequence is identical to that of mouse origin
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 228

• Applicable Applications for anti-RYBP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB (0.5 - 1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RYBP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: RYBP antibody was raised against synthetic peptide from human RYBP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (93%).
• Antigen Modification: aa215-228
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-RYBP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-RYBP antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of DEDAF in A549 cells with DEDAF antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 ug /ml.)

Related Product Information for anti-RYBP antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and -10, and FADD was identified recently and designated DEDAF for DED associated factor.

Product Categories/Family for anti-RYBP antibody

Family: Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 112363092
• NCBI GeneID: 23429
• NCBI Accession #: NP_036366.3
• NCBI GenBank Nucleotide #: NM_012234.6
• UniProt Accession #: Q8N488; Q9P2W5; Q9UMW4
• Molecular Weight: 24,822 Da
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: RYBP
• NCBI Official Synonym Symbols: AAP1; DEDAF; YEAF1
• NCBI Protein Information: RING1 and YY1-binding protein; APAP-1; DED-associated factor; ring1 interactor RYBP; apoptin-associating protein 1; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; death effector domain-associated factor
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RYBP
• UniProt Synonym Gene Names: DEDAF; YEAF1; APAP-1; DED-associated factor
• UniProt Entry Name: RYBP_HUMAN

Uniprot Description

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter