Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat RUTBC3 Polyclonal Antibody | anti-SGSM3 antibody
Goat anti-RUTBC3 Antibody
Gene Names
SGSM3; MAP; RUSC3; RUTBC3; RABGAP5; RabGAP-5; rabGAPLP
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RUTBC3; Polyclonal Antibody; Goat anti-RUTBC3 Antibody; SGSM3; small G protein signaling modulator 3; DKFZp761D051; MAP; RUSC3; RUN and SH3 containing 3; RUN and TBC1 domain containing 3; merlin binding protein; merlin-associated protein; small G protein signaling modulator 3 protein; SGSM3 antibody; small G protein signaling modulator 3 antibody; DKFZp761D051 antibody; MAP antibody; RUSC3 antibody; RUTBC3 antibody; RUN and SH3 containing 3 antibody; RUN and TBC1 domain containing 3 antibody; merlin binding protein antibody; merlin-associated protein antibody; small G protein signaling modulator 3 protein antibody; anti-SGSM3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RDHENYVACSRSHRR
Sequence Length
749
Applicable Applications for anti-SGSM3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx.85kDa band observed in lysates of Human Lung carcinoma cell line A549 (calculated MW of 85.4kDa according to NP_056520.2). Recommended concentration: 2-3ug/ml.
Western Blot: Approx.85kDa band observed in lysates of Human Lung carcinoma cell line A549 (calculated MW of 85.4kDa according to NP_056520.2). Recommended concentration: 2-3ug/ml.
Immunogen
Peptide with sequence RDHENYVACSRSHRR, from the internal region of the protein sequence according to NP_056520.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,308 Da
NCBI Official Full Name
small G protein signaling modulator 3 isoform 1
NCBI Official Synonym Full Names
small G protein signaling modulator 3
NCBI Official Symbol
SGSM3
NCBI Official Synonym Symbols
MAP; RUSC3; RUTBC3; RABGAP5; RabGAP-5; rabGAPLP
NCBI Protein Information
small G protein signaling modulator 3
UniProt Protein Name
Small G protein signaling modulator 3
Protein Family
UniProt Gene Name
SGSM3
UniProt Synonym Gene Names
RabGAPLP
UniProt Entry Name
SGSM3_HUMAN
Uniprot Description
SGSM3: May play a cooperative role in NF2-mediated growth suppression of cells. Belongs to the small G protein signaling modulator family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein regulator, misc.
Chromosomal Location of Human Ortholog: 22q13.1-q13.2
Cellular Component: cytosol; endomembrane system
Molecular Function: GTPase activator activity; protein binding; Rab GTPase binding
Biological Process: cell cycle arrest; intracellular protein transport; plasma membrane to endosome transport; Rap protein signal transduction; regulation of Rab protein signal transduction; regulation of vesicle fusion
Research Articles on SGSM3
Similar Products
Product Notes
The SGSM3 sgsm3 (Catalog #AAA422117) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RUTBC3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's RUTBC3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx.85kDa band observed in lysates of Human Lung carcinoma cell line A549 (calculated MW of 85.4kDa according to NP_056520.2). Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the SGSM3 sgsm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RDHENYVACS RSHRR. It is sometimes possible for the material contained within the vial of "RUTBC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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