Rabbit DDA Polyclonal Antibody | anti-DDA antibody

DDA Antibody

Cat. Num. AAA9403928

• Gene Names: SEC62; HTP1; TP-1; Dtrp1; TLOC1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: DDA; Polyclonal Antibody; DDA Antibody; DET1 and DDB1 associated 1; PCIA1; anti-DDA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 399

• Applicable Applications for anti-DDA antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against an 18 amino acid peptide near the carboxy terminus of human DDA1.
• Target Name: DDA
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide)

Related Product Information for anti-DDA antibody

DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.

Product Categories/Family for anti-DDA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507525
• NCBI GeneID: 7095
• NCBI Accession #: NP_003253
• NCBI GenBank Nucleotide #: NM_003262.3
• Molecular Weight: 45,862 Da
• NCBI Official Full Name: translocation protein SEC62
• NCBI Official Synonym Full Names: SEC62 homolog, preprotein translocation factor
• NCBI Official Symbol: SEC62
• NCBI Official Synonym Symbols: HTP1; TP-1; Dtrp1; TLOC1
• NCBI Protein Information: translocation protein SEC62
• UniProt Protein Name: Translocation protein SEC62
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: SEC62
• UniProt Synonym Gene Names: TLOC1; TP-1; hTP-1
• UniProt Entry Name: SEC62_HUMAN

NCBI Description

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]

Uniprot Description

SEC62: a multi-pass membrane protein and component of the Sec61 complex of the rough endoplasmic reticulum membrane. The Sec61 complex may perform post-translational protein translocation into the ER and backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway.

Protein type: Channel, misc.; Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3q26.2

Cellular Component: endoplasmic reticulum membrane; intermediate filament cytoskeleton; rough endoplasmic reticulum; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: receptor activity; protein transporter activity

Biological Process: posttranslational protein targeting to membrane; cotranslational protein targeting to membrane