Testing Data
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-1.jpgAll lanes: Anti RUNX1 (MBS177470) at 0.5ug/mlWB: Recombinant Human RUNX1 Protein 0.5ngPredicted bind size: 50KDObserved bind size: 50KD )
Rabbit Runt-related transcription factor 1 Polyclonal Antibody | anti-RUNX1 antibody
Anti-RUNX1/AML1 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Testing Data
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-1.jpgAll lanes: Anti RUNX1 (MBS177470) at 0.5ug/mlWB: Recombinant Human RUNX1 Protein 0.5ngPredicted bind size: 50KDObserved bind size: 50KD )
Testing Data
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-1.jpgAll lanes: Anti RUNX1 (MBS177470) at 0.5ug/mlWB: Recombinant Human RUNX1 Protein 0.5ngPredicted bind size: 50KDObserved bind size: 50KD )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-2.JPGIHC(P): Rat Thymus Tissue )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-2.JPGIHC(P): Rat Thymus Tissue )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-3.JPGIHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-3.JPGIHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-4.JPGIHC(P): Mouse Intestine Tissue )
Immunohistochemistry (IHC)
(Anti-RUNX1/AML1 Picoband antibody, MBS177470-4.JPGIHC(P): Mouse Intestine Tissue )
Background: Runt-related transcription factor 1 (RUNX1), also known as AML1 or CBFA2, is a protein that in humans is encoded by the RUNX1 gene. It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-alpha (CBFalpha). RUNX1 is mapped to 21q22.12. RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. RUNX proteins form a heterodimeric complex with CBFbeta which confers increased DNA binding and stability to the complex. Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. Mutations in RUNX1 are implicated in cases of breast cancer.
NCBI and Uniprot Product Information
NCBI Description
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
AML1: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize. Interacts with TLE1 and ALYREF/THOC4. Interacts with ELF1, ELF2 and SPI1. Interacts via its Runt domain with the ELF4 N-terminal region. Interaction with ELF2 isoform 2 (NERF-1a) may act to repress RUNX1-mediated transactivation. Interacts with KAT6A and KAT6B. Interacts with SUV39H1, leading to abrogation of transactivating and DNA-binding properties of RUNX1. Interacts with YAP1. Interacts with HIPK2. Interaction with CDK6 prevents myeloid differentiation, reducing its transcription transactivation activity. Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood. 11 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; basement membrane; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; calcium ion binding; transcription factor binding; transcription factor activity; ATP binding
Biological Process: central nervous system development; hair follicle morphogenesis; transcription, DNA-dependent; embryonic hemopoiesis; in utero embryonic development; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; regulation of signal transduction; liver development; negative regulation of granulocyte differentiation; peripheral nervous system neuron development; behavioral response to pain; positive regulation of angiogenesis; myeloid progenitor cell differentiation; positive regulation of interleukin-2 production; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; skeletal development
Disease: Platelet Disorder, Familial, With Associated Myeloid Malignancy; Leukemia, Acute Myeloid; Rheumatoid Arthritis
Research Articles on RUNX1
Similar Products
Product Notes
The RUNX1 runx1 (Catalog #AAA177470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RUNX1/AML1 Antibody reacts with Human, Mouse, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's Runt-related transcription factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human; Predicted Species: Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat. Researchers should empirically determine the suitability of the RUNX1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Runt-related transcription factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
