Rabbit RTKN Polyclonal Antibody | anti-RTKN antibody

RTKN Polyclonal Antibody

Cat. Num. AAA3007290

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RTKN; Polyclonal Antibody; RTKN Polyclonal Antibody; Rhotekin; RTKN1; anti-RTKN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RTKN polyclonal antibody detects endogenous levels of RTKN protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 563

• Applicable Applications for anti-RTKN antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RTKN.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RTKN polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate Lane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysateLane4:Hela whole cell lysate)

Related Product Information for anti-RTKN antibody

Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.

NCBI and Uniprot Product Information

• NCBI GI #: 62739179
• NCBI GeneID: 6242
• NCBI Accession #: NP_001015055.1
• NCBI GenBank Nucleotide #: NP_001015055.1
• UniProt Accession #: Q9BST9
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: rhotekin isoform a
• NCBI Official Synonym Full Names: rhotekin
• NCBI Official Symbol: RTKN
• NCBI Protein Information: rhotekin
• UniProt Protein Name: Rhotekin
• Protein Family: Rhotekin
• UniProt Gene Name: RTKN
• UniProt Entry Name: RTKN_HUMAN

NCBI Description

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

RTKN: Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein regulator, misc.

Chromosomal Location of Human Ortholog: 2p13.1

Molecular Function: protein binding; GTP binding; GTP-Rho binding; GTPase inhibitor activity

Biological Process: apoptosis; negative regulation of catalytic activity; signal transduction; Rho protein signal transduction