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Western Blot (WB) (RT4I1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RT4I1 antibody detected the RT4I1 protein (arrow).)

Rabbit anti-Human RT4I1 Polyclonal Antibody | anti-RTN4IP1 antibody

RT4I1 Antibody (C-term)

Gene Names
RTN4IP1; NIMP
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RT4I1; Polyclonal Antibody; RT4I1 Antibody (C-term); Reticulon-4-interacting protein 1; mitochondrial; NOGO-interacting mitochondrial protein; RTN4IP1; NIMP; anti-RTN4IP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RT4I1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the C-terminal region of human RT4I1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
335-363
Sequence Length
396
Applicable Applications for anti-RTN4IP1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RT4I1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RT4I1 antibody detected the RT4I1 protein (arrow).)

Western Blot (WB) (RT4I1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RT4I1 antibody detected the RT4I1 protein (arrow).)

Immunohistochemistry (IHC)

(RT4I1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT4I1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RT4I1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT4I1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RT4I1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (RT4I1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RTN4IP1 antibody
This gene encodes a novel mitochondrial protein that
interacts with reticulon 4, which is a potent inhibitor of
regeneration following spinal cord injury. The interaction of
reticulon 4 with mitochondrial proteins may provide insight into
the mechanisms for reticulon-induced inhibition of neurite growth.
Product Categories/Family for anti-RTN4IP1 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Domeniconi, M., et al. Neuron 35(2):283-290(2002)
Hu, W.H., et al. J. Neurochem. 81(1):36-45(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43590
NCBI Official Full Name
reticulon-4-interacting protein 1, mitochondrial
NCBI Official Synonym Full Names
reticulon 4 interacting protein 1
NCBI Official Symbol
RTN4IP1
NCBI Official Synonym Symbols
NIMP
NCBI Protein Information
reticulon-4-interacting protein 1, mitochondrial
UniProt Protein Name
Reticulon-4-interacting protein 1, mitochondrial
UniProt Gene Name
RTN4IP1
UniProt Synonym Gene Names
NIMP
UniProt Entry Name
RT4I1_HUMAN

NCBI Description

This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq, Jul 2008]

Uniprot Description

RTN4IP1: Appears to be a potent inhibitor of regeneration following spinal cord injury. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: mitochondrion

Molecular Function: zinc ion binding; oxidoreductase activity

Research Articles on RTN4IP1

Similar Products

Product Notes

The RTN4IP1 rtn4ip1 (Catalog #AAA9211734) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 335-363. The RT4I1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RT4I1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the RTN4IP1 rtn4ip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RT4I1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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