Rabbit anti-Human RT4I1 Polyclonal Antibody | anti-RT4I1 antibody

RT4I1, CT (RTN4IP1, NIMP, Reticulon-4-interacting protein 1, mitochondrial, NOGO-interacting mitochondrial protein) (Azide free) (HRP)

Cat. Num. AAA6344297

• Gene Names: RTN4IP1; NIMP
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RT4I1; Polyclonal Antibody; CT (RTN4IP1; NIMP; Reticulon-4-interacting protein 1; mitochondrial; NOGO-interacting mitochondrial protein) (Azide free) (HRP); RTN4IP1; NOGO-interacting mitochondrial protein; anti-RT4I1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RT4I1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RT4I1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 338-367 amino acids from the C-terminal region of human RT4I1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RT4I1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RT4I1 antibody detected the RT4I1 protein (arrow).)

Immunohistochemistry (IHC)

(RT4I1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT4I1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RT4I1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RT4I1 antibody

This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth.

Product Categories/Family for anti-RT4I1 antibody

Antibodies; Mitochondrial Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Mungall, A.J., et al. Nature 425(6960):805-811(2003). Domeniconi, M., et al. Neuron 35(2):283-290(2002). Hu, W.H., et al. J. Neurochem. 81(1):36-45(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 47519420
• NCBI GeneID: 84816
• NCBI Accession #: NP_116119.2
• NCBI GenBank Nucleotide #: NM_032730.4
• UniProt Accession #: Q8WWV3; Q8N9B3; Q8WZ66; Q9BRA4
• Molecular Weight: 24,936 Da
• NCBI Official Full Name: reticulon-4-interacting protein 1, mitochondrial
• NCBI Official Synonym Full Names: reticulon 4 interacting protein 1
• NCBI Official Symbol: RTN4IP1
• NCBI Official Synonym Symbols: NIMP
• NCBI Protein Information: reticulon-4-interacting protein 1, mitochondrial; NOGO-interacting mitochondrial protein
• UniProt Protein Name: Reticulon-4-interacting protein 1, mitochondrial
• UniProt Gene Name: RTN4IP1
• UniProt Synonym Gene Names: NIMP
• UniProt Entry Name: RT4I1_HUMAN

NCBI Description

This gene encodes a novel mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. The interaction of reticulon 4 with mitochondrial proteins may provide insight into the mechanisms for reticulon-induced inhibition of neurite growth. [provided by RefSeq, Jul 2008]

Uniprot Description

RTN4IP1: Appears to be a potent inhibitor of regeneration following spinal cord injury. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: mitochondrion

Molecular Function: zinc ion binding; oxidoreductase activity