Rabbit anti-Human, Mouse RT34 Polyclonal Antibody | anti-RT34 antibody

RT34, NT (MRPS34, 28S ribosomal protein S34, mitochondrial) (Biotin)

Cat. Num. AAA6344284

• Gene Names: MRPS34; MRPS12; MRP-S12; MRP-S34
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RT34; Polyclonal Antibody; NT (MRPS34; 28S ribosomal protein S34; mitochondrial) (Biotin); MRPS34; mitochondrial; anti-RT34 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RT34 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RT34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human RT34.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RT34 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RT34 antibody detected the RT34 protein (arrow).)

Flow Cytometry (FC/FACS)

(RT34 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RT34 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined.

Product Categories/Family for anti-RT34 antibody

Antibodies; Mitochondrial Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Zhang, Z., et al. Genomics 81(5):468-480(2003). Ogawa, F., et al. Biochem. Biophys. Res. Commun. 300(3):789-792(2003). Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001). O'Brien, T.W., et al. J. Biol. Chem. 275(24):18153-18159(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 13027604
• NCBI GeneID: 65993
• NCBI Accession #: NP_076425.1
• NCBI GenBank Nucleotide #: NM_023936.1
• UniProt Accession #: P82930; Q9BVI7
• Molecular Weight: 25,650 Da
• NCBI Official Full Name: 28S ribosomal protein S34, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S34
• NCBI Official Symbol: MRPS34
• NCBI Official Synonym Symbols: MRPS12; MRP-S12; MRP-S34
• NCBI Protein Information: 28S ribosomal protein S34, mitochondrial; S34mt
• UniProt Protein Name: 28S ribosomal protein S34, mitochondrial
• UniProt Gene Name: MRPS34
• UniProt Synonym Gene Names: MRP-S34; S34mt
• UniProt Entry Name: RT34_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS34: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis