Immunofluorescence (IF)
(MBS9602231 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse Rsk-4 Polyclonal Antibody | anti-Rsk-4 antibody
Rsk-4 Antibody
Gene Names
RPS6KA6; RSK4; RSK-4; p90RSK6; PP90RSK4; S6K-alpha-6
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Rsk-4; Polyclonal Antibody; Rsk-4 Antibody; 90 kDa ribosomal protein S6 kinase 6; KS6A6_HUMAN; p90 RSK 6; p90-RSK 6; p90RSK6; pp90RSK4; Ribosomal protein S6 kinase 90kDa polypeptide 6; Ribosomal protein S6 kinase alpha 6; Ribosomal protein S6 kinase alpha-6; Ribosomal S6 kinase 4; rps6ka6; RSK 4; RSK-4; S6K-alpha-6; anti-Rsk-4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Rsk-4 antibody detects endogenous levels of total Rsk-4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
745
Applicable Applications for anti-Rsk-4 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Rsk-4
Subcellular Location
Nucleus; Cytosol
Predicted Cross Reactivity
Pig, Horse, Rabbit
Similarity
Pig (90%), Horse (80%), Rabbit (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602231 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602231 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of Rsk-4 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Rsk-4 using K562 whole cell lysates)
Related Product Information for anti-Rsk-4 antibody
Function: Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.
Subunit Structure: Forms a complex with MAPK3/ERK1 but not with MAPK9 or MAPK14 in serum-starved cells.
Post-translational Modifications: Phosphorylated at Ser-232, Ser-372, and Ser-389 in serum-starved cells.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Subunit Structure: Forms a complex with MAPK3/ERK1 but not with MAPK9 or MAPK14 in serum-starved cells.
Post-translational Modifications: Phosphorylated at Ser-232, Ser-372, and Ser-389 in serum-starved cells.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 84 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
ribosomal protein S6 kinase alpha-6 isoform 1
NCBI Official Synonym Full Names
ribosomal protein S6 kinase A6
NCBI Official Symbol
RPS6KA6
NCBI Official Synonym Symbols
RSK4; RSK-4; p90RSK6; PP90RSK4; S6K-alpha-6
NCBI Protein Information
ribosomal protein S6 kinase alpha-6
UniProt Protein Name
Ribosomal protein S6 kinase alpha-6
UniProt Gene Name
RPS6KA6
UniProt Synonym Gene Names
RSK4; S6K-alpha-6; p90-RSK 6; p90RSK6; RSK-4
NCBI Description
This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]
Uniprot Description
Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.
Research Articles on Rsk-4
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Product Notes
The Rsk-4 rps6ka6 (Catalog #AAA9602231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rsk-4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rsk-4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the Rsk-4 rps6ka6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rsk-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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