Western Blot (WB)
(POLR2G Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).)
Rabbit POLR2G Polyclonal Antibody | anti-POLR2G antibody
POLR2G Antibody (C-term)
Western Blot (WB)
(POLR2G Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).)
Western Blot (WB)
(POLR2G Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the POLR2G antibody detected the POLR2G protein (arrow).)
Flow Cytometry (FC/FACS)
(POLR2G Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(POLR2G Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
polymerase II, the polymerase responsible for synthesizing
messenger RNA in eukaryotes. The protein functions in transcription
initiation, and is also thought to help stabilize transcribing
polyermase molecules during elongation.
Ujvari, A., et al. Nat. Struct. Mol. Biol. 13(1):49-54(2006)
Meka, H., et al. Nucleic Acids Res. 33(19):6435-6444(2005)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671(2003)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the seventh largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq, Jan 2009]
Uniprot Description
POLR2G: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Binds RNA. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: nucleoplasm; nucleolus; DNA-directed RNA polymerase II, core complex; nucleus
Molecular Function: single-stranded RNA binding; translation initiation factor binding; DNA-directed RNA polymerase activity; single-stranded DNA binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; somatic stem cell maintenance; positive regulation of viral transcription; apoptosis; mRNA catabolic process, exonucleolytic; RNA splicing; DNA repair; nuclear mRNA splicing, via spliceosome; positive regulation of translational initiation; mRNA capping; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression
Research Articles on POLR2G
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Product Notes
The POLR2G polr2g (Catalog #AAA9204247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 126-153. The POLR2G Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the POLR2G polr2g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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