Western Blot (WB)
(Western blot analysis of Rsk-3 using HeLa whole cell lysates)
Rabbit anti-Human, Mouse Rsk-3 Polyclonal Antibody | anti-Rsk-3 antibody
Rsk-3 Antibody
Gene Names
RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Rsk-3; Polyclonal Antibody; Rsk-3 Antibody; 90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK2; pp90RSK3; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase; 90kDa; polypeptide 2; Ribosomal S6 kinase 3; RPS6KA2; RSK 3; RSK; RSK-3; S6K alpha; S6K alpha 2; S6K-alpha-2; anti-Rsk-3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Rsk-3 antibody detects endogenous levels of total Rsk-3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
741
Applicable Applications for anti-Rsk-3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human Rsk-3
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Rsk-3 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of Rsk-3 using HeLa whole cell lysates)
Related Product Information for anti-Rsk-3 antibody
Function: Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Subunit Structure: Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity).
Post-translational Modifications: Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity). N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Subunit Structure: Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity).
Post-translational Modifications: Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity). N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 83 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
ribosomal protein S6 kinase alpha-2 isoform b
NCBI Official Synonym Full Names
ribosomal protein S6 kinase A2
NCBI Official Symbol
RPS6KA2
NCBI Official Synonym Symbols
RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
NCBI Protein Information
ribosomal protein S6 kinase alpha-2
UniProt Protein Name
Ribosomal protein S6 kinase alpha-2
UniProt Gene Name
RPS6KA2
UniProt Synonym Gene Names
MAPKAPK1C; RSK3; S6K-alpha-2; p90-RSK 2; p90RSK2; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; RSK-3
NCBI Description
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2016]
Uniprot Description
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Research Articles on Rsk-3
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Product Notes
The Rsk-3 rps6ka2 (Catalog #AAA9608920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rsk-3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rsk-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the Rsk-3 rps6ka2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rsk-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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