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ELISA (EIA) (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rsk-3 (Phospho-Thr353+T356) Antibody)

Rabbit anti-Human, Mouse Rsk-3 Polyclonal Antibody | anti-Rsk-3 antibody

Rsk-3 (Phospho-Thr353+Thr356) Antibody

Gene Names
RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Rsk-3; Polyclonal Antibody; Rsk-3 (Phospho-Thr353+Thr356) Antibody; anti-Rsk-3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Rsk-3 (Phospho-Thr353+Thr356) antibody detects endogenous levels of Rsk-3 only when phosphorylated at Thr353+Thr356.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
741
Applicable Applications for anti-Rsk-3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
Target Modification
Phospho
Modification Sites
H:T353+T356; M:T353+T356
Immunogen
The antiserum was produced against synthesized phospho-peptide derived from human Rsk-3 around the phosphorylation site of Thr353+Thr356.
Preparation and Storage
Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rsk-3 (Phospho-Thr353+T356) Antibody)

ELISA (EIA) (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rsk-3 (Phospho-Thr353+T356) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells, using p-Rsk-3 (Phospho-Thr353+T356) antibody.)

Western Blot (WB) (Western blot analysis of lysates from HeLa cells, using p-Rsk-3 (Phospho-Thr353+T356) antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83 kDa
NCBI Official Full Name
ribosomal protein S6 kinase alpha-2 isoform b
NCBI Official Synonym Full Names
ribosomal protein S6 kinase A2
NCBI Official Symbol
RPS6KA2
NCBI Official Synonym Symbols
RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
NCBI Protein Information
ribosomal protein S6 kinase alpha-2
UniProt Protein Name
Ribosomal protein S6 kinase alpha-2
UniProt Gene Name
RPS6KA2
UniProt Synonym Gene Names
MAPKAPK1C; RSK3; S6K-alpha-2; p90-RSK 2; p90RSK2; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; RSK-3

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2016]

Uniprot Description

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.

Research Articles on Rsk-3

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Product Notes

The Rsk-3 rps6ka2 (Catalog #AAA9508545) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rsk-3 (Phospho-Thr353+Thr356) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rsk-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the Rsk-3 rps6ka2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rsk-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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