Rabbit RPS6KB2 Polyclonal Antibody | anti-RPS6KB2 antibody

RPS6KB2 (Ribosomal Protein S6 Kinase Beta 2, KLS, SRK, S6K2, STK14B, p70S6Kb,-beta, S6K-beta2, P70-beta-1, P70-beta-2, p70(S6K)-beta) (APC)

Cat. Num. AAA6436320

• Gene Names: RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: RPS6KB2; Polyclonal Antibody; RPS6KB2 (Ribosomal Protein S6 Kinase Beta 2; KLS; SRK; S6K2; STK14B; p70S6Kb; -beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta) (APC); anti-RPS6KB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes p70S6Kb. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-RPS6KB2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human p70S6Kb.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cell line, using P70S6Kb antibody.)

Product Categories/Family for anti-RPS6KB2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 55953117
• NCBI GeneID: 6199
• NCBI Accession #: NP_003943.2
• NCBI GenBank Nucleotide #: NM_003952.2
• UniProt Accession #: Q9UBS0; O94809; Q9UEC1; B2RMZ9
• Molecular Weight: 53,455 Da
• NCBI Official Full Name: ribosomal protein S6 kinase beta-2
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 70kDa, polypeptide 2
• NCBI Official Symbol: RPS6KB2
• NCBI Official Synonym Symbols: KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
• NCBI Protein Information: ribosomal protein S6 kinase beta-2; P70S6K2; S6K-beta; p70 S6KB; p70-S6K 2; S6K-beta-2; p70 S6K-beta; p70 S6 kinase beta; S6 kinase-related kinase; p70 ribosomal S6 kinase beta; serine/threonine-protein kinase 14B; 70 kDa ribosomal protein S6 kinase 2; se
• UniProt Protein Name: Ribosomal protein S6 kinase beta-2
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KB2
• UniProt Synonym Gene Names: STK14B; S6K-beta-2; S6K2; P70S6K2; p70-S6K 2; SRK; S6K-beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70-beta
• UniProt Entry Name: KS6B2_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

P70S6KB: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Translation; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein serine/threonine kinase activity; ribosomal protein S6 kinase activity; peptide binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of translational initiation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; translation; nerve growth factor receptor signaling pathway; protein kinase B signaling cascade; innate immune response; signal transduction; protein amino acid phosphorylation