Rabbit RPS6KB1 Polyclonal Antibody | anti-RPS6KB1 antibody

RPS6KB1, phosphorylated (Thr421) (Ribosomal Protein S6 Kinase beta-1, S6K-beta-1, S6K1, 70kD Ribosomal Protein S6 Kinase 1, P70S6K1, p70-S6K 1, p70 Ribosomal S6 Kinase alpha, p70 S6 Kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K, Ribosomal Protein S6 Kina

Cat. Num. AAA623522

• Gene Names: RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RPS6KB1; Polyclonal Antibody; phosphorylated (Thr421) (Ribosomal Protein S6 Kinase beta-1; S6K-beta-1; S6K1; 70kD Ribosomal Protein S6 Kinase 1; P70S6K1; p70-S6K 1; p70 Ribosomal S6 Kinase alpha; p70 S6 Kinase alpha; p70 S6K-alpha; p70 S6KA; p70-S6K; Ribosomal Protein S6 Kina; Anti -RPS6KB1; anti-RPS6KB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS6KB1 when phosphorylated at threonine 421. Species Cross-Reactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-RPS6KB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot:1:500-1:1,000; Immunohistochemistry:1:50-1:100
• Immunogen: Synthetic phosphopeptide corresponding to human RPS6KB1 around the phosphorylation site of threonine 421 (P-R-TP-P-V).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-RPS6KB1 antibody

RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.

Product Categories/Family for anti-RPS6KB1 antibody

Antibodies; Abs to Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 4506737
• NCBI GeneID: 6198
• NCBI Accession #: NP_003152.1
• NCBI GenBank Nucleotide #: NM_003161.2
• UniProt Accession #: P23443; Q4G0X7; Q7Z721
• Molecular Weight: 59,140 Da
• NCBI Official Full Name: ribosomal protein S6 kinase beta-1
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• NCBI Official Symbol: RPS6KB1
• NCBI Official Synonym Symbols: S6K; PS6K; S6K1; STK14A; p70-S6K; p70-alpha; p70(S6K)-alpha
• NCBI Protein Information: ribosomal protein S6 kinase beta-1; P70S6K1; p70 S6KA; S6K-beta-1; p70 S6K-alpha; OTTHUMP00000198109; OTTHUMP00000198110; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; ribosomal protein S6 kinase I; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; 70 kDa ribosomal protein S6 kinase 1
• UniProt Protein Name: Ribosomal protein S6 kinase beta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KB1
• UniProt Synonym Gene Names: STK14A
• UniProt Entry Name: KS6B1_HUMAN

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq]

Uniprot Description

p70S6K: an AGC kinase of the RSK family that is required for cell growth and G1 cell cycle progression. Is phosphorylated and activated by mTOR in mitogenic pathways downstream of phosphoinositide 3 kinase (PI3K). Phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. Activity is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains. Mouse knockout shows symptoms of insulin resistance, and increased insulin senstivity, resulting in protection against diet-induced obesity. Protein expression and activation upregulated in colon adenocarcinoma cell lines. Increased expression in breast cancer correlated with poor survival. Selectively amplified and overexpressed within the 17q23 breast cancer amplicon. Two isoforms produced by alternative initiation have been reported.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Translation; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; p70 subfamily

Chromosomal Location of Human Ortholog: 17q23.1