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Immunohistochemistry (IHC) (Anti-MSK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Rabbit anti-Human, Mouse RPS6KA5 / MSK1 Polyclonal Antibody | anti-RPS6KA5 antibody

Rabbit Polyclonal (IgG) to Human RPS6KA5 / MSK1

Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Immunoaffinity Purified
Synonyms
RPS6KA5 / MSK1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human RPS6KA5 / MSK1; Anti-RPS6KA5 / MSK1 Antibody (aa331-380) IHC-plus; RPS6KA5; MSPK1; RLPK; MSK1; RSK-like protein kinase; RSKL; S6K-alpha-5; Human RPS6KA5; anti-RPS6KA5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MSK1 antibody detects endogenous levels of total MSK1 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
802
Applicable Applications for anti-RPS6KA5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA (1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
Target Species
Human
Immunogen Description
Synthetic peptide from human MSK1 (aa331-380).
Immunogen
RPS6KA5 / MSK1 antibody was raised against synthetic peptide from human MSK1 (aa331-380).
Antigen Modification
aa331-380
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-MSK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-MSK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from COS7 cells, treated with Sorbitol 0.4 M 30', using MSK1 antibody. The lane on the right is treated with the synthesized peptide.)

Western Blot (WB) (Western blot of extracts from COS7 cells, treated with Sorbitol 0.4 M 30', using MSK1 antibody. The lane on the right is treated with the synthesized peptide.)
Related Product Information for anti-RPS6KA5 antibody
MSK1 is a mitogen- and stress-activated protein kinase that belongs to the MSK family. In vivo, the MSKs act as ERK MAPK and p38 MAPK substrates. MSK1, and likely MSK2, localize to the nucleus and are strong CREB kinases.
Product Categories/Family for anti-RPS6KA5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61,769 Da
NCBI Official Full Name
ribosomal protein S6 kinase alpha-5 isoform a
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 5
NCBI Official Symbol
RPS6KA5
NCBI Official Synonym Symbols
MSK1; RLPK; MSPK1
NCBI Protein Information
ribosomal protein S6 kinase alpha-5; RSKL; S6K-alpha-5; RSK-like protein kinase; 90 kDa ribosomal protein S6 kinase 5; nuclear mitogen- and stress-activated protein kinase 1
UniProt Protein Name
Ribosomal protein S6 kinase alpha-5
UniProt Gene Name
RPS6KA5
UniProt Synonym Gene Names
MSK1; S6K-alpha-5; RSKL
UniProt Entry Name
KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; positive regulation of histone phosphorylation; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; histone phosphorylation; stress-activated MAPK cascade; activation of CREB transcription factor; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; negative regulation of cytokine production; toll-like receptor signaling pathway; innate immune response; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; negative regulation of transcription, DNA-dependent; toll-like receptor 4 signaling pathway

Research Articles on RPS6KA5

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Product Notes

The RPS6KA5 rps6ka5 (Catalog #AAA244267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human RPS6KA5 / MSK1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6KA5 / MSK1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:1000). Researchers should empirically determine the suitability of the RPS6KA5 rps6ka5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6KA5 / MSK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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