Rabbit RPS6KA3 Polyclonal Antibody | anti-RPS6KA3 antibody

RPS6KA3 (S369) antibody

Cat. Num. AAA9231234

• Gene Names: RPS6KA3; CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• Reactivity: Human, Mouse; Predicted: Zebrafish
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: RPS6KA3; Polyclonal Antibody; RPS6KA3 (S369) antibody; Purified Rabbit Polyclonal Antibody (Pab); Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 2.7.11.1; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2; ISPK1; MAPKAPK1B; RSK2; anti-RPS6KA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 342-376 amino acids from human.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 740

• Applicable Applications for anti-RPS6KA3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Tissue Location: Expressed in many tissues, highest levels in skeletal muscle
• Cellular Location: Nucleus. Cytoplasm
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RPS6KA3-pS369. ctrl at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: Hela whole cell lysateLane 5: HepG2 whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-RPS6KA3 (S369) antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: Hela whole cell lysateLane 5: HepG2 whole cell lysateLane 6: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RPS6KA3 antibody

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR- independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro- apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA- EPHA2 signaling pathway controls cell migration (PubMed:26158630).

Product Categories/Family for anti-RPS6KA3 antibody

Primary Antibodies; Signal Transduction

References

Bjoerbaek C.,et al.Diabetes 44:90-97(1995). Ota T.,et al.Nat. Genet. 36:40-45(2004). Totoki Y.,et al. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. Ross M.T.,et al.Nature 434:325-337(2005). Moller D.E.,et al.Am. J. Physiol. 266:C351-C359(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 4759050
• NCBI GeneID: 6197
• NCBI Accession #: NP_004577.1
• NCBI GenBank Nucleotide #: NM_004586.2
• UniProt Accession #: P51812; Q4VAP3; Q59H26; Q5JPK8; Q7Z3Z7; B2R9V4
• Molecular Weight: 83736
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A3
• NCBI Official Symbol: RPS6KA3
• NCBI Official Synonym Symbols: CLS; RSK; HU-3; RSK2; MRX19; ISPK-1; p90-RSK2; pp90RSK2; MAPKAPK1B; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA3
• UniProt Synonym Gene Names: ISPK1; MAPKAPK1B; RSK2; S6K-alpha-3; p90-RSK 3; p90RSK3; ISPK-1; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).