Western Blot (WB)
(Western blot analysis of VCAN using HUVEC whole cell lysates)
Rabbit anti-Human VCAN Polyclonal Antibody | anti-VCAN antibody
VCAN Antibody
Gene Names
VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
VCAN; Polyclonal Antibody; VCAN Antibody; Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan core protein; cartilage; CSPG2; CSPG2_HUMAN; ERVR; GHAP; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PGM; Versican; Versican core protein; Versican proteoglycan; WGN 1; WGN; WGN1; anti-VCAN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
VCAN antibody detects endogenous levels of total VCAN
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
655
Applicable Applications for anti-VCAN antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human VCAN
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.
Predicted Cross Reactivity
Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Bovine (91%), Sheep (91%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of VCAN using HUVEC whole cell lysates)
Western Blot (WB)
(Western blot analysis of VCAN using HUVEC whole cell lysates)
Related Product Information for anti-VCAN antibody
Function: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Subunit Structure: Interacts with FBLN1.
Post-translational Modifications: Phosphorylated by FAM20C in the extracellular medium.
Similarity: Belongs to the aggrecan/versican proteoglycan family.
Subunit Structure: Interacts with FBLN1.
Post-translational Modifications: Phosphorylated by FAM20C in the extracellular medium.
Similarity: Belongs to the aggrecan/versican proteoglycan family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 373 kDa
Predicted: 373 kDa
Predicted: 373 kDa
NCBI Official Full Name
versican core protein isoform 2
NCBI Official Synonym Full Names
versican
NCBI Official Symbol
VCAN
NCBI Official Synonym Symbols
WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
NCBI Protein Information
versican core protein
UniProt Protein Name
Versican core protein
Protein Family
UniProt Gene Name
VCAN
UniProt Synonym Gene Names
CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
NCBI Description
This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Uniprot Description
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Research Articles on VCAN
Similar Products
Product Notes
The VCAN vcan (Catalog #AAA9602866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VCAN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VCAN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the VCAN vcan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VCAN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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