Rabbit anti-Human, Mouse RPS6 Polyclonal Antibody | anti-RPS6 antibody

RPS6 (Ser240/244) (RPS6, 40S ribosomal protein S6, Phosphoprotein NP33) (FITC)

Cat. Num. AAA6343878

• Gene Names: RPS6; S6
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS6; Polyclonal Antibody; RPS6 (Ser240/244) (RPS6; 40S ribosomal protein S6; Phosphoprotein NP33) (FITC); Phosphoprotein NP33; anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RPS6 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-257 amino acids from human RPS6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS6 (arrow) using rabbit polyclonal RPS6 (Ser240/244) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2).)

Western Blot (WB)

(RPS6 (Ser240/244) Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the RPS6 antibody detected the RPS6 protein (arrow).)

Western Blot (WB)

(RPS6 (Ser240/244) Antibody western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the RPS6 antibody detected the RPS6 protein (arrow).)

Immunohistochemistry (IHC)

(RPS6 antibody (Ser240/244) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPS6 Antibody (Ser240/244) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPS6 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPS6 antibody

Antibodies; Ribosomal Proteins

References

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008). Fujita, K., et al. Acta Neuropathol. 116(4):439-445(2008). Robledo, S., et al. RNA 14(9):1918-1929(2008). Glover, E.I., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295 (2), R604-R610 (2008): Ma, X.M., et al. Cell 133(2):303-313(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 17158044
• NCBI GeneID: 6194
• NCBI Accession #: NP_001001.2
• NCBI GenBank Nucleotide #: NM_001010.2
• UniProt Accession #: P62753; P08227; P10660; Q4VBY7; Q8N6Z7
• Molecular Weight: 28,681 Da
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: RPS6
• NCBI Official Synonym Symbols: S6
• NCBI Protein Information: 40S ribosomal protein S6; phosphoprotein NP33
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS6
• UniProt Entry Name: RS6_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: protein binding; structural constituent of ribosome; protein kinase binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; erythrocyte development; TOR signaling pathway; translation; viral reproduction; positive regulation of apoptosis; T cell differentiation in the thymus; gastrulation; translational termination; viral infectious cycle; glucose homeostasis; oogenesis stage; T cell proliferation during immune response; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; activated T cell apoptosis; placenta development; mitosis; mitotic cell cycle checkpoint; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; mRNA catabolic process, nonsense-mediated decay; insulin receptor signaling pathway; gene expression; rRNA processing; G1/S transition of mitotic cell cycle; negative regulation of apoptosis