Western Blot (WB)
(Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysatesLane 2: rat spleen tissue lysatesLane 3: mouse HEPA1-6 wholel cell lysatesLane 4: mouse SP2/0 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: human U-87MG whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human PC-3 whole cell lysatesLane 9: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # MBS1753627) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD. )
Rabbit Arp2/ACTR2 Polyclonal Antibody | anti-ACTR2 antibody
Anti-Arp2/ACTR2 Antibody
IHC-P: 0.5-1ug/ml|Human|
ICC/IF: 4ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysatesLane 2: rat spleen tissue lysatesLane 3: mouse HEPA1-6 wholel cell lysatesLane 4: mouse SP2/0 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: human U-87MG whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human PC-3 whole cell lysatesLane 9: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # MBS1753627) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysatesLane 2: rat spleen tissue lysatesLane 3: mouse HEPA1-6 wholel cell lysatesLane 4: mouse SP2/0 whole cell lysatesLane 5: monkey COS-7 whole cell lysatesLane 6: human U-87MG whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human PC-3 whole cell lysatesLane 9: human U20S whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # MBS1753627) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Arp2/ACTR2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Arp2/ACTR2 Antibody (MBS1753627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Arp2/ACTR2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Arp2/ACTR2 Antibody (MBS1753627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunofluorescence (IF)
(Figure 3. IF analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Arp2/ACTR2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-Arp2/ACTR2 Antibody (MBS1753627) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 3. IF analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (MBS1753627).Arp2/ACTR2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-Arp2/ACTR2 Antibody (MBS1753627) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of A431 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing A431 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 4. Flow Cytometry analysis of A431 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing A431 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of ANA-1 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing ANA-1 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of ANA-1 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing ANA-1 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 6. Flow Cytometry analysis of C6 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing C6 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 6. Flow Cytometry analysis of C6 cells using anti-Arp2/ACTR2 antibody (MBS1753627).Overlay histogram showing C6 cells stained with MBS1753627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arp2/ACTR2 Antibody (MBS1753627,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Hanisch, J., Kolm, R., Wozniczka, M., Bumann, D., Rottner, K., Stradal, T. E. B. Activation of a RhoA/myosin II-dependent but Arp2/3 complex-independent pathway facilitates Salmonella invasion. Cell Host Microbe 9: 273-285, 2011.
3. Li, P., Banjade, S., Cheng, H. -C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., Russo, P. S., Jiang, Q. -X., Nixon, B. T., Rosen, M. K. Phase transitions in the assembly of multivalent signalling proteins. Nature 483: 336-340, 2012.
NCBI and Uniprot Product Information
NCBI Description
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ARP2: Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Belongs to the actin family. ARP2 subfamily.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: Arp2/3 protein complex; cell projection; focal adhesion; membrane; cytoplasm; cytosol; actin cap; actin cytoskeleton
Molecular Function: actin filament binding; structural constituent of cytoskeleton; ATP binding
Biological Process: meiotic chromosome movement towards spindle pole; axon guidance; asymmetric cell division; ephrin receptor signaling pathway; establishment and/or maintenance of cell polarity; innate immune response; cilium biogenesis; cytokinesis after meiosis; cell motility; cytoplasmic transport; spindle localization
Research Articles on ACTR2
Similar Products
Product Notes
The ACTR2 actr2 (Catalog #AAA1753627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Arp2/ACTR2 Antibody reacts with Human, Mouse, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Arp2/ACTR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Monkey, Rat| IHC-P: 0.5-1ug/ml|Human| ICC/IF: 4ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human, Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the ACTR2 actr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arp2/ACTR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
