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Western Blot (WB) (Western blot analysis of RPS6 Antibody (N-term) in HL60 cell line lysates (35ug/lane). RPS6 (arrow) was detected using the purified Pab.)

Rabbit anti-Human RPS6 Polyclonal Antibody | anti-rpsF antibody

RPS6, NT (40S Ribosomal Protein S6, Phosphoprotein NP33, OK/SW-cl.2)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
RPS6; Polyclonal Antibody; NT (40S Ribosomal Protein S6; Phosphoprotein NP33; OK/SW-cl.2); Anti -RPS6; anti-rpsF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RPS6.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-rpsF antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50
Immunogen
Synthetic peptide selected from the N-terminal region of human RPS6 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS6 Antibody (N-term) in HL60 cell line lysates (35ug/lane). RPS6 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RPS6 Antibody (N-term) in HL60 cell line lysates (35ug/lane). RPS6 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RPS6 (arrow) using rabbit polyclonal RPS6 Antibody (N-term) (RB12889). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2) (Origene Technologies).)

Western Blot (WB) (Western blot analysis of RPS6 (arrow) using rabbit polyclonal RPS6 Antibody (N-term) (RB12889). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-rpsF antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS6 is a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA.
Product Categories/Family for anti-rpsF antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14,505 Da
NCBI Official Full Name
RpS6
NCBI Official Symbol
rpsF
NCBI Protein Information
30S ribosomal protein S6
UniProt Protein Name
30S ribosomal protein S6
Protein Family
UniProt Gene Name
rpsF
UniProt Synonym Gene Names
rps6
UniProt Entry Name
RS6_PASMU

Uniprot Description

Function: Binds together with S18 to 16S ribosomal RNA

By similarity. HAMAP-Rule MF_00360

Sequence similarities: Belongs to the ribosomal protein S6P family.

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Product Notes

The rpsF rpsf (Catalog #AAA6004384) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS6, NT (40S Ribosomal Protein S6, Phosphoprotein NP33, OK/SW-cl.2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPS6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50. Researchers should empirically determine the suitability of the rpsF rpsf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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