Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: HepG2 cells, Jurkat cells, Hela cells, Raji cells, RAW264.7 cells, A549 cells, 293T cells, Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Rabbit anti-Human, Mouse RPS3 Polyclonal Antibody | anti-RPS3 antibody
RPS3 Antibody
Immunohistochemistry: 1:25-1:100
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: HepG2 cells, Jurkat cells, Hela cells, Raji cells, RAW264.7 cells, A549 cells, 293T cells, Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-7: HepG2 cells, Jurkat cells, Hela cells, Raji cells, RAW264.7 cells, A549 cells, 293T cells, Primary antibody: 1/350 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/25,)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/25,)
NCBI and Uniprot Product Information
Uniprot Description
RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer.
Protein type: Apoptosis; EC 4.2.99.18; RNA-binding; Ribosomal; Translation
Chromosomal Location of Human Ortholog: 11q13.3-q13.5
Cellular Component: cytoplasm; cytosol; extracellular matrix; focal adhesion; membrane; mitochondrial inner membrane; mitochondrial matrix; nucleolus; nucleoplasm; nucleus; plasma membrane; polysome; ribonucleoprotein complex; ribosome
Molecular Function: damaged DNA binding; DNA binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; endodeoxyribonuclease activity; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; kinase binding; microtubule binding; mRNA binding; oxidized purine DNA binding; oxidized pyrimidine DNA binding; protein binding; protein kinase A binding; protein kinase binding; RNA binding; structural constituent of ribosome; SUMO binding; transcription factor binding; tubulin binding
Biological Process: chromosome segregation; DNA damage response, detection of DNA damage; mRNA catabolic process, nonsense-mediated decay; negative regulation of DNA repair; negative regulation of protein ubiquitination; negative regulation of translation; positive regulation of DNA repair; positive regulation of endodeoxyribonuclease activity; positive regulation of JNK activity; positive regulation of microtubule polymerization; regulation of apoptosis; response to DNA damage stimulus; response to oxidative stress; rRNA processing; spindle assembly; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription
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Product Notes
The RPS3 rps3 (Catalog #AAA9416312) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RPS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the RPS3 rps3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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