Rabbit anti-Human, Rat RPS27 Polyclonal Antibody | anti-RPS27 antibody

RPS27 Polyclonal Antibody

Cat. Num. AAA3011810

• Gene Names: RPS27; S27; MPS1; DBA17; MPS-1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPS27; Polyclonal Antibody; RPS27 Polyclonal Antibody; 40S ribosomal protein S27; Metallopan-stimulin 1; MPS-1; MPS1; anti-RPS27 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPS27 polyclonal antibody detects endogenous levels of RPS27 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 84

• Applicable Applications for anti-RPS27 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human RPS27.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using RPS27 antibody.)

Related Product Information for anti-RPS27 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 4506711
• NCBI GeneID: 6232
• NCBI Accession #: NP_001021.1
• NCBI GenBank Nucleotide #: NP_001021.1
• UniProt Accession #: P42677
• Molecular Weight: ~ 9kDa
• NCBI Official Full Name: 40S ribosomal protein S27 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S27
• NCBI Official Symbol: RPS27
• NCBI Official Synonym Symbols: S27; MPS1; DBA17; MPS-1
• NCBI Protein Information: 40S ribosomal protein S27
• UniProt Protein Name: 40S ribosomal protein S27
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS27
• UniProt Synonym Gene Names: MPS1; MPS-1

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain that can bind to zinc and may bind to nucleic acid. Mutations in this gene have been identified in numerous melanoma patients and in at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2018]

Uniprot Description

RPS27: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; rRNA processing; sister chromatid cohesion; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription