Rabbit RPS20 Polyclonal Antibody | anti-RPS20 antibody

RPS20 Polyclonal Antibody

Cat. Num. AAA3013377

• Gene Names: RPS20; S20; uS10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: RPS20; Polyclonal Antibody; RPS20 Polyclonal Antibody; 40S ribosomal protein S20; Ribosomal protein S20; RPS 20; RpS20; RS20; S20; anti-RPS20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPS20 polyclonal antibody detects endogenous levels of RPS20 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 119

• Applicable Applications for anti-RPS20 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of RPS20 on different lysates using anti-RPS20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431)

Immunocytochemistry (ICC)

(ICC staining RPS20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-RPS20 antibody

Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5 and 3 minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.

NCBI and Uniprot Product Information

• NCBI GI #: 4506697
• NCBI GeneID: 6224
• NCBI Accession #: NP_001014.1
• NCBI GenBank Nucleotide #: NP_001014.1
• UniProt Accession #: P60866; P60867; P60868
• Molecular Weight: 13kDa
• NCBI Official Full Name: 40S ribosomal protein S20 isoform 2
• NCBI Official Synonym Full Names: ribosomal protein S20
• NCBI Official Symbol: RPS20
• NCBI Official Synonym Symbols: S20; uS10
• NCBI Protein Information: 40S ribosomal protein S20
• UniProt Protein Name: 40S ribosomal protein S20
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS20
• UniProt Entry Name: RS20_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

RPS20: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q12

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination