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Western Blot (WB) (Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)

Rabbit anti-Human RPS15 Polyclonal Antibody | anti-RPS15 antibody

RPS15, NT (40S Ribosomal Protein S15, RIG Protein, RIG) (APC)

Gene Names
RPS15; RIG; S15
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
RPS15; Polyclonal Antibody; NT (40S Ribosomal Protein S15; RIG Protein; RIG) (APC); anti-RPS15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RPS15.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-RPS15 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human RPS15 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of RPS15 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). RPS15 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-RPS15 antibody
References
1. Yu, Y., et.al., Protein Sci. 14(6), 1438-1446 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,040 Da
NCBI Official Full Name
40S ribosomal protein S15
NCBI Official Synonym Full Names
ribosomal protein S15
NCBI Official Symbol
RPS15
NCBI Official Synonym Symbols
RIG; S15
NCBI Protein Information
40S ribosomal protein S15; insulinoma protein; homolog of rat insulinoma
UniProt Protein Name
40S ribosomal protein S15
Protein Family
UniProt Gene Name
RPS15
UniProt Synonym Gene Names
RIG
UniProt Entry Name
RS15_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS15: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Ribosomal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; focal adhesion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; DNA binding

Biological Process: ribosomal small subunit export from nucleus; SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; translational termination; viral infectious cycle; osteoblast differentiation; ribosomal small subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; viral transcription; gene expression; rRNA processing

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Product Notes

The RPS15 rps15 (Catalog #AAA6497741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS15, NT (40S Ribosomal Protein S15, RIG Protein, RIG) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPS15 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RPS15 rps15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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