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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,MCF-7 whole cell lysateAll lanes: RPS15 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 18 KDaObserved band size: 18 KDa)

Rabbit anti-Human RPS15 Polyclonal Antibody | anti-RPS15 antibody

RPS15 Antibody

Gene Names
RPS15; RIG; S15
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
RPS15; Polyclonal Antibody; RPS15 Antibody; 40S ribosomal protein S15; RIG protein; RIG; anti-RPS15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
145
Applicable Applications for anti-RPS15 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human 40S ribosomal protein S15 protein (63-137AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate,MCF-7 whole cell lysateAll lanes: RPS15 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 18 KDaObserved band size: 18 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,MCF-7 whole cell lysateAll lanes: RPS15 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 18 KDaObserved band size: 18 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7049044 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MBS7049044 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS7049044 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen tissue using MBS7049044 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7049044 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7049044 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-RPS15 antibody
cytoplasm, cytosol, cytosolic small ribosomal subunit, focal adhesion, membrane, nucleoplasm, nucleus, DNA binding, poly(A) RNA binding, structural constituent of ribosome

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,040 Da
NCBI Official Full Name
40S ribosomal protein S15 isoform 2
NCBI Official Synonym Full Names
ribosomal protein S15
NCBI Official Symbol
RPS15
NCBI Official Synonym Symbols
RIG; S15
NCBI Protein Information
40S ribosomal protein S15
UniProt Protein Name
40S ribosomal protein S15
Protein Family
UniProt Gene Name
RPS15
UniProt Synonym Gene Names
RIG
UniProt Entry Name
RS15_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

RPS15: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19P family of ribosomal proteins. It is located in the cytoplasm. This gene has been found to be activated in various tumors, such as insulinomas, esophageal cancers, and colon cancers. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Ribosomal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; osteoblast differentiation; ribosomal small subunit assembly and maintenance; ribosomal small subunit biogenesis and assembly; ribosomal small subunit export from nucleus; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription

Research Articles on RPS15

Similar Products

Product Notes

The RPS15 rps15 (Catalog #AAA7049044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS15 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPS15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the RPS15 rps15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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