HSD17B10 recombinant protein

HSD17B10, 12-261aa, Human, His tag, E Coli

Cat. Num. AAA203699

• Gene Names: HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; HSD10MD; 17b-HSD10; DUPXp11.22
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HSD17B10; 12-261aa; Human; His tag; E Coli; 3-hydroxyacyl-CoA dehydrogenase type-2; Hydroxyacyl Coenzyme A dehydrogenase type II; Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase; 17 beta hydroxysteroid dehydrogenase 10; 17 beta hydroxysteroid dehydrogenase type 10; 17b HSD10; 3 hydroxy 2 methylbutyryl CoA dehydrogenase; 3 hydroxyacyl CoA dehydrogenase type 2; 3 hydroxyacyl CoA dehydrogenase type II; AB binding alcohol dehydrogenase; ABAD; Ads9; Amyloid beta binding polypeptide; Amyloid beta peptide binding alcohol dehydrogenase; Amyloid beta peptide binding protein; CAMR; DuPXp11.22; Endoplasmic Reticulum Amyloid Binding Protein; Endoplasmic reticulum associated amyloid beta peptide binding protein; ER associated amyloid beta-binding protein; ERAB; HADH 2; HADH2; HCD 2; HCD2; Hydroxyacyl CoA Dehydrogenase type II; Hydroxysteroid (17 beta) dehydrogenase 10; Mental retardation X linked syndromic 11; MHBD; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; MRPP2; MRX17; SCHAD; SDR5C1; Short chain dehydrogenase/reductase family 5C member 1; Short chain L 3 hydroxyacyl CoA dehydrogenase type 2; Short chain type dehydrogenase/reductase XH98G2; Type 10 17b HSD; Type 10 17beta hydroxysteroid dehydrogenase; Type II HADH; XH98G2; HSD17B10 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, and 100 mM NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MVAVITGGAS GLGLATAERL VGQGASAVLL DLPNSGGEAQ AKKLGNNCVF APADVTSEKD VQTALALAKG KFGRVDVAVN CAGIAVASKT YNLKKGQTHT LEDFQRVLDV NLMGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVMTIAPGL FGTPLLTSLP EKVCNFLASQ VPFPSRLGDP AEYAHLVQAI IENPFLNGEV IRLDGAIRMQ P
• Sequence Length: 261

• Applicable Applications for HSD17B10 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HSD17B10 recombinant protein

HSD17B10 is a member of the short-chain dehydrogenase/reductase superfamily. This mitochondrial protein catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. HSD17B10 plays an important role in processing steroid hormones and fats, and also helps break down the protein building block (amino acid) isoleucine. This enzyme is also necessary for several chemical reactions involving female sex hormones (estrogens) and male sex hormones (androgens). It is essential for maintaining appropriate levels of male and female sex hormones. This protein may contribute to the neuronal dysfunction associated with Alzheimer disease. Recombinant HSD17B10 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for HSD17B10 recombinant protein

Redox Proteins

References

Hoizmann J., et al. (2008) Cell. 135 (3):462-74. ; Yan SD., et al. (1997) Nature. 389 (6652):689-95. ;

NCBI and Uniprot Product Information

• NCBI GI #: 4758504
• NCBI GeneID: 3028
• NCBI Accession #: NP_004484
• NCBI GenBank Nucleotide #: NM_004493.2
• UniProt Accession #: Q99714; Q5H927; Q6IBS9; Q8TCV9; Q96HD5
• Molecular Weight: 28.1 kDa (271aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: 3-hydroxyacyl-CoA dehydrogenase type-2 isoform 1
• NCBI Official Synonym Full Names: hydroxysteroid 17-beta dehydrogenase 10
• NCBI Official Symbol: HSD17B10
• NCBI Official Synonym Symbols: ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; HSD10MD; 17b-HSD10; DUPXp11.22
• NCBI Protein Information: 3-hydroxyacyl-CoA dehydrogenase type-2
• UniProt Protein Name: 3-hydroxyacyl-CoA dehydrogenase type-2
• Protein Family: 3-hydroxyacyl-CoA dehydrogenase
• UniProt Gene Name: HSD17B10
• UniProt Synonym Gene Names: ERAB; HADH2; MRPP2; SCHAD; SDR5C1; XH98G2; Mitochondrial RNase P protein 2

NCBI Description

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]

Uniprot Description

Mitochondrial dehydrogenase that catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone (PubMed:9553139, PubMed:23042678, PubMed:12917011, PubMed:18996107, PubMed:25925575, PubMed:28888424). Catalyzes the third step in the beta-oxidation of fatty acids (PubMed:9553139, PubMed:12917011, PubMed:18996107, PubMed:25925575, PubMed:28888424). Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids (PubMed:12917011). Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids (PubMed:12917011). By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) (PubMed:9338779). Essential for structural and functional integrity of mitochondria (PubMed:20077426).