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Western Blot (WB) (All lanes : Anti-RPS10 Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateLane 5: C6 whole cell lysateLane 6: mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit RPS10 Polyclonal Antibody | anti-RPS10 antibody

RPS10 Antibody (C-Term)

Gene Names
RPS10; S10; DBA9
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Bovine
Applications
Western Blot, ELISA
Synonyms
RPS10; Polyclonal Antibody; RPS10 Antibody (C-Term); 40S ribosomal protein S10; anti-RPS10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat; Predicted Reactivity: Bovine
Clonality
Polyclonal
Isotype
Ig
Specificity
This RPS10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-129 amino acids from human RPS10.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
165
Applicable Applications for anti-RPS10 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB:~~1:2000
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasm. Nucleus, nucleolus. Note=Localized in the granular component (GC) region of the nucleolus Methylation is required for its localization in the GC region Colocalizes with NPS1 in the GC region of the nucleolus.
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RPS10 Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateLane 5: C6 whole cell lysateLane 6: mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-RPS10 Antibody (C-Term) at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: K562 whole cell lysateLane 5: C6 whole cell lysateLane 6: mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RPS10 antibody
Component of the 40S ribosomal subunit.
Product Categories/Family for anti-RPS10 antibody
References
Frigerio J.-M.,et al.Biochim. Biophys. Acta 1262:64-68 (1995). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Mungall A.J.,et al.Nature 425:805-811 (2003). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Vladimirov S.N.,et al.Eur. J. Biochem. 239:144-149 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18898 Da
NCBI Official Full Name
40S ribosomal protein S10
NCBI Official Synonym Full Names
ribosomal protein S10
NCBI Official Symbol
RPS10
NCBI Official Synonym Symbols
S10; DBA9
NCBI Protein Information
40S ribosomal protein S10
UniProt Protein Name
40S ribosomal protein S10
Protein Family
UniProt Gene Name
RPS10
UniProt Entry Name
RS10_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011]

Uniprot Description

RPS10: a ribosomal protein of the S10E family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 40S subunit.

Protein type: Translation; Nucleolus; Ribosomal; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription

Disease: Diamond-blackfan Anemia 9

Research Articles on RPS10

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Product Notes

The RPS10 rps10 (Catalog #AAA9217193) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPS10 Antibody (C-Term) reacts with Human, Mouse, Rat; Predicted Reactivity: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's RPS10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB:~~1:2000. Researchers should empirically determine the suitability of the RPS10 rps10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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