Rabbit anti-Human RPP25 Polyclonal Antibody | anti-RPP25 antibody

RPP25, CT (RPP25, Ribonuclease P protein subunit p25) (Biotin)

Cat. Num. AAA6343668

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPP25; Polyclonal Antibody; CT (RPP25; Ribonuclease P protein subunit p25) (Biotin); Ribonuclease P protein subunit p25; anti-RPP25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RPP25 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the C-terminal region of human RPP25.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPP25 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RPP25 antibody detected the RPP25 protein (arrow).)

Related Product Information for anti-RPP25 antibody

Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.

Product Categories/Family for anti-RPP25 antibody

Antibodies; Enzymes; Ribonuclease

References

Hands-Taylor, K.L., et al. Nucleic Acids Res. 38(12):4052-4066(2010). Lamesch, P., et al. Genomics 89(3):307-315(2007). Welting, T.J., et al. Nucleic Acids Res. 32(7):2138-2146(2004). Guerrier-Takada, C., et al. RNA 8(3):290-295(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 93277074
• NCBI GeneID: 54913
• NCBI Accession #: NP_060263.2
• NCBI GenBank Nucleotide #: NM_017793.2
• UniProt Accession #: Q9BUL9; Q9NX88; D3DW70
• Molecular Weight: 20,632 Da
• NCBI Official Full Name: ribonuclease P protein subunit p25
• NCBI Official Synonym Full Names: ribonuclease P/MRP 25kDa subunit
• NCBI Official Symbol: RPP25
• NCBI Protein Information: ribonuclease P protein subunit p25; RNase P protein subunit p25; ribonuclease P 25kDa subunit
• UniProt Protein Name: Ribonuclease P protein subunit p25
• Protein Family: Ribonuclease P protein
• UniProt Gene Name: RPP25
• UniProt Synonym Gene Names: RNase P protein subunit p25
• UniProt Entry Name: RPP25_HUMAN

Uniprot Description

Function: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA.

Catalytic activity: Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.

Subunit structure: Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a RNA moiety and at least 8 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38 and RPP40. Interacts with the P3 domain of RNase MRP complex. RNase MRP consists of a RNA moiety and at least 9 protein subunits; POP1, RPP14, RPP20/POP7, RPP25, RPP29/POP4, RPP30, RPP38, RPP40, POP5 and RPP21. Ref.5

Subcellular location: Nucleus Ref.1.

Sequence similarities: Belongs to the histone-like Alba family.