Western Blot (WB)
(Western blot analysis of lysates from PC-3,U-251 MG cell line (from left to right), using RPLP0P6Antibody(N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Rabbit RPLP0P6 Polyclonal Antibody | anti-RPLP0P6 antibody
RPLP0P6 Antibody (N-term)
Reactivity
HumanPredicted: Bovine, Chicken, Zebrafish, Mouse, Pig, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
RPLP0P6; Polyclonal Antibody; RPLP0P6 Antibody (N-term); 60S acidic ribosomal protein P0-like; anti-RPLP0P6 antibody
Host
Rabbit
Reactivity
Human
Predicted: Bovine, Chicken, Zebrafish, Mouse, Pig, Rat
Predicted: Bovine, Chicken, Zebrafish, Mouse, Pig, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This RPLP0P6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPLP0P6.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
317
Applicable Applications for anti-RPLP0P6 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:1000
IF: 1:25
IHC-P: 1:25
IF: 1:25
IHC-P: 1:25
Antigen Source
Human
Antigen Region
1-30
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from PC-3,U-251 MG cell line (from left to right), using RPLP0P6Antibody(N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from PC-3,U-251 MG cell line (from left to right), using RPLP0P6Antibody(N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with RPLP0P6 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with RPLP0P6 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. stomach section using RPLP0P6 Antibody (N-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded H. stomach section using RPLP0P6 Antibody (N-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-RPLP0P6 antibody
Description: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Background: Ribosomal protein P0 is the functional equivalent of E.coli protein L10 (By similarity).
Background: Ribosomal protein P0 is the functional equivalent of E.coli protein L10 (By similarity).
References
Gorry M.C., et al. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases. Li W.B., et al. Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases. Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Gauci S., et al. Anal. Chem. 81:4493-4501(2009).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
34364
NCBI Official Full Name
60S acidic ribosomal protein P0-like
UniProt Protein Name
60S acidic ribosomal protein P0-like
Protein Family
UniProt Gene Name
RPLP0P6
Uniprot Description
Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
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Product Notes
The RPLP0P6 rplp0p6 (Catalog #AAA9231691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPLP0P6 Antibody (N-term) reacts with Human Predicted: Bovine, Chicken, Zebrafish, Mouse, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPLP0P6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:1000 IF: 1:25 IHC-P: 1:25. Researchers should empirically determine the suitability of the RPLP0P6 rplp0p6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPLP0P6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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