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SDS-Page (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-6: HepG2 cell£¬Mouse brain tissue£¬Jurkat£¬MCF-7£¬A172 and Raji cell, Primary antibody:RPL8 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Rabbit RPL8 Polyclonal Antibody | anti-RPL8 antibody

RPL8 Antibody

Gene Names
RPL8; L8
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Synonyms
RPL8; Polyclonal Antibody; RPL8 Antibody; L8; anti-RPL8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total RPL8 protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.2mg/ml (varies by lot)
Sequence Length
257
Applicable Applications for anti-RPL8 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Western Blot: 1:200-1000
Immunohistochemistry: 1: 10-50
Immunogen
Fusion protein of human RPL8
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

SDS-Page

(Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-6: HepG2 cell£¬Mouse brain tissue£¬Jurkat£¬MCF-7£¬A172 and Raji cell, Primary antibody:RPL8 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

SDS-Page (Gel: 12%SDS-PAGE Lysate: 40 ug, Lane 1-6: HepG2 cell£¬Mouse brain tissue£¬Jurkat£¬MCF-7£¬A172 and Raji cell, Primary antibody:RPL8 antibody at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RPL8 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using RPL8 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200))
Related Product Information for anti-RPL8 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Product Categories/Family for anti-RPL8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,025 Da
NCBI Official Full Name
60S ribosomal protein L8
NCBI Official Synonym Full Names
ribosomal protein L8
NCBI Official Symbol
RPL8
NCBI Official Synonym Symbols
L8
NCBI Protein Information
60S ribosomal protein L8
UniProt Protein Name
60S ribosomal protein L8
Protein Family
UniProt Gene Name
RPL8
UniProt Entry Name
RL8_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL8: a ribosomal protein of the L2P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Associates with 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface.

Protein type: RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus

Molecular Function: RNA binding

Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription

Research Articles on RPL8

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Product Notes

The RPL8 rpl8 (Catalog #AAA9416980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPL8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPL8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Western Blot: 1:200-1000 Immunohistochemistry: 1: 10-50. Researchers should empirically determine the suitability of the RPL8 rpl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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