Rabbit anti-Human RPL5 Polyclonal Antibody | anti-RPL5 antibody

RPL5, NT (RPL5, 60S ribosomal protein L5) (AP)

Cat. Num. AAA6343567

• Gene Names: RPL5; L5; DBA6; MSTP030
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RPL5; Polyclonal Antibody; NT (RPL5; 60S ribosomal protein L5) (AP); 60S ribosomal protein L5; anti-RPL5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPL5.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPL5 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human RPL5, UniProt Accession #P46777. Species sequence homology: Xenopus, bovine, chicken, monkey, porcine and rabbit
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6002994 in Y79 cell line lysates (35ug/lane). RPL5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS6002994 in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002994 for immunohistochemistry.)

Related Product Information for anti-RPL5 antibody

RPL5 encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II.

Product Categories/Family for anti-RPL5 antibody

Antibodies; Ribosomal Proteins

References

1. Quarello, P., et.al., Haematologica 95 (2), 206-213 (2010). 2. Hoppenbrouwers, I.A., et.al., J. Hum. Genet. 54 (11), 676-680 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14591909
• NCBI GeneID: 6125
• NCBI Accession #: NP_000960.2
• NCBI GenBank Nucleotide #: NM_000969.3
• UniProt Accession #: P46777; Q32LZ3; Q53HH6; Q9H3F4
• Molecular Weight: 34,363 Da
• NCBI Official Full Name: 60S ribosomal protein L5
• NCBI Official Synonym Full Names: ribosomal protein L5
• NCBI Official Symbol: RPL5
• NCBI Official Synonym Symbols: L5; DBA6; MSTP030
• NCBI Protein Information: 60S ribosomal protein L5
• UniProt Protein Name: 60S ribosomal protein L5
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL5
• UniProt Entry Name: RL5_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. This gene is co-transcribed with the small nucleolar RNA gene U21, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL5: Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA. Defects in RPL5 are the cause of Diamond-Blackfan anemia type 6 (DBA6). DBA6 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L18P family.

Protein type: Nucleolus; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: focal adhesion; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding; 5S rRNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; translational termination; ribosomal large subunit biogenesis and assembly; viral infectious cycle; translational elongation; cellular protein metabolic process; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; rRNA processing

Disease: Diamond-blackfan Anemia 6