Rabbit RPL35 Polyclonal Antibody | anti-RPL35 antibody

RPL35 (M91) Polyclonal Antibody

Cat. Num. AAA3004308

• Gene Names: RPL35; L35; DBA19
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPL35; Polyclonal Antibody; RPL35 (M91) Polyclonal Antibody; 60S ribosomal protein L35; L35; Ribosomal protein L35; RL35; anti-RPL35 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ribosomal Protein L35 (M91) polyclonal antibody detects endogenous levels of Ribosomal Protein L35 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 123

• Applicable Applications for anti-RPL35 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids 70-120 of Human Ribosomal Protein L35.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPL35 (M91) pAb at 1:500 dilutionLane1:Hela whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:The Ovary tissue lysate of Rat (20ug) Lane4:The Kidney tissue lysate of Mouse (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RPL35 (M91) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Immunoprecipitation (IP)

(Immunoprecipitation of HEK293T cell lysate using RPL35  (M91) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3). Lane 1 is 30% input. The Western Blot was probed using RPL35  (M91) # MBS3004308. “up-arrow” (supernatant) ; “down-arrow (deposition))

Related Product Information for anti-RPL35 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 6005860
• NCBI GeneID: 11224
• NCBI Accession #: NP_009140.1
• NCBI GenBank Nucleotide #: NP_009140.1
• UniProt Accession #: P42766
• Molecular Weight: ~ 15kDa
• NCBI Official Full Name: 60S ribosomal protein L35
• NCBI Official Synonym Full Names: ribosomal protein L35
• NCBI Official Symbol: RPL35
• NCBI Official Synonym Symbols: L35; DBA19
• NCBI Protein Information: 60S ribosomal protein L35
• UniProt Protein Name: 60S ribosomal protein L35
• Protein Family: 50S ribosomal protein
• UniProt Gene Name: RPL35
• UniProt Entry Name: RL35_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL35: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: mRNA binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination