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Western Blot (WB) ((0.1ug/ml) staining of Human Colorectal Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat RPL23 Polyclonal Antibody | anti-RPL23 antibody

Goat anti-RPL23 Antibody

Gene Names
RPL23; L23; rpL17
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RPL23; Polyclonal Antibody; Goat anti-RPL23 Antibody; ribosomal protein L23; L23; MGC111167; MGC117346; MGC72008; rpL17; 60S ribosomal protein L17; 60S ribosomal protein L23; OTTHUMP00000164151; OTTHUMP00000164153; ribosomal protein L17; 60S ribosomal protein L17 antibody; 60S ribosomal protein L23 antibody; L23 antibody; MGC111167 antibody; MGC117346 antibody; MGC72008 antibody; OTTHUMP00000164151 antibody; OTTHUMP00000164153 antibody; ribosomal protein L17 antibody; ribosomal protein L23 antibody; rpL17 antibody; RPL23 antibody; anti-RPL23 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KECADLWPRIASN
Sequence Length
140
Applicable Applications for anti-RPL23 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence KECADLWPRIASN, from the C Terminus of the protein sequence according to NP_000969.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Colorectal Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Colorectal Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,865 Da
NCBI Official Full Name
60S ribosomal protein L23
NCBI Official Synonym Full Names
ribosomal protein L23
NCBI Official Symbol
RPL23
NCBI Official Synonym Symbols
L23; rpL17
NCBI Protein Information
60S ribosomal protein L23
UniProt Protein Name
60S ribosomal protein L23
Protein Family
UniProt Gene Name
RPL23
UniProt Entry Name
RL23_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL17 because the encoded protein shares amino acid identity with ribosomal protein L17 from Saccharomyces cerevisiae; however, its official symbol is RPL23. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL23: a ribosomal protein of the L14P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 17q

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; ribosome

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal protein import into nucleus; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription

Research Articles on RPL23

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Product Notes

The RPL23 rpl23 (Catalog #AAA422966) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RPL23 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's RPL23 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the RPL23 rpl23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KECADLWPRI ASN. It is sometimes possible for the material contained within the vial of "RPL23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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