Rabbit anti-Human, Mouse RPL15 Polyclonal Antibody | anti-RPL15 antibody

RPL15, NT (RPL15, EC45, 60S ribosomal protein L15) (APC)

Cat. Num. AAA6343370

• Gene Names: RPL15; L15; EC45; DBA12; RPL10; RPLY10; RPYL10
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL15; Polyclonal Antibody; NT (RPL15; EC45; 60S ribosomal protein L15) (APC); 60S ribosomal protein L15; anti-RPL15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 204

• Applicable Applications for anti-RPL15 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPL15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-58 amino acids from the N-terminal region of human RPL15.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6005967. This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) usingMBS6005967.This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6005967 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of K562 cells (right histogram) using MBS6005967 compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPL15 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL15 antibody

Antibodies; Ribosomal Proteins

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Wang, H., et al. BMC Cancer 6, 91 (2006): Andersen, J.S., et al. Nature 433(7021):77-83(2005). Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004): Mazumder, B., et al. Cell 115(2):187-198(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 15431293
• NCBI GeneID: 6138
• NCBI Accession #: NP_002939.2
• NCBI GenBank Nucleotide #: NP_002939.2
• UniProt Accession #: P61313
• NCBI Official Full Name: 60S ribosomal protein L15 isoform 1
• NCBI Official Synonym Full Names: ribosomal protein L15
• NCBI Official Symbol: RPL15
• NCBI Official Synonym Symbols: L15; EC45; DBA12; RPL10; RPLY10; RPYL10
• NCBI Protein Information: 60S ribosomal protein L15
• UniProt Protein Name: 60S ribosomal protein L15
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL15
• UniProt Synonym Gene Names: EC45
• UniProt Entry Name: RL15_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L15E family of ribosomal proteins and a component of the 60S subunit. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Elevated expression of this gene has been observed in esophageal tumors and gastric cancer tissues, and deletion of this gene has been observed in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]

Uniprot Description

RPL15: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Nov 2011]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 3p24.2

Cellular Component: ribosome; cytosol; nucleus

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination

Disease: Diamond-blackfan Anemia 12