Rabbit anti-Human RPL10 Polyclonal Antibody | anti-RPL10 antibody

RPL10, NT (RPL10, DXS648E, QM, 60S ribosomal protein L10, Laminin receptor homolog, Protein QM, Tumor suppressor QM) (Azide free) (HRP)

Cat. Num. AAA6343307

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL10; Polyclonal Antibody; NT (RPL10; DXS648E; QM; 60S ribosomal protein L10; Laminin receptor homolog; Protein QM; Tumor suppressor QM) (Azide free) (HRP); Tumor suppressor QM; anti-RPL10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 214

• Applicable Applications for anti-RPL10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human RPL10.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPL10 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the RPL10 antibody detected the RPL10 protein (arrow).)

Related Product Information for anti-RPL10 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Transcript variants utilizing alternative polyA signals exist. The variant with the longest 3' UTR overlaps the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPL10 antibody

Antibodies; Lamin; Laminin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Gong, X., et al. BMC Med. Genet. 10, 7 (2009): Nishimura, M., et al. J. Mol. Biol. 377(2):421-430(2008). Farmer, A.A., et al. Nucleic Acids Res. 24(11):2158-2165(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 223890243
• NCBI Accession #: NP_006004.2
• NCBI GenBank Nucleotide #: NP_006004.2
• UniProt Accession #: P27635
• NCBI Official Full Name: 60S ribosomal protein L10 isoform a
• UniProt Protein Name: 60S ribosomal protein L10
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL10
• UniProt Synonym Gene Names: DXS648E; QM
• UniProt Entry Name: RL10_HUMAN

Uniprot Description

RPL10: Defects in RPL10 are a cause of susceptibility to autism X-linked type 5 (AUTSX5). A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. RPL10 is involved in autism only in rare cases. Two hypomorphic variants affecting the translation process have been found in families with autism spectrum disorders, suggesting that aberrant translation may play a role in disease mechanisms. Belongs to the ribosomal protein L10e family.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: membrane; endoplasmic reticulum; cytosol

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; viral infectious cycle; translational termination

Disease: Autism, Susceptibility To, X-linked 5