Rabbit anti-Human, Rat RPGRIP1L Polyclonal Antibody | anti-RPGRIP1L antibody

RPGRIP1L Antibody

Cat. Num. AAA9603399

• Gene Names: RPGRIP1L; FTM; MKS5; CORS3; JBTS7; NPHP8; PPP1R134
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RPGRIP1L; Polyclonal Antibody; RPGRIP1L Antibody; CORS 3; CORS3; Fantom; FTM; FTM_HUMAN; JBTS 1; JBTS 7; JBTS1; JBTS7; Joubert syndrome 1; Joubert syndrome 7; Meckel syndrome; type 1; type 5; MKS 5; MKS5; Nephrocystin-8; NPHP 8; NPHP8; Protein fantom; Retinitis pigmentosa GTPase regulator interacting protein 1 like; RPGR interacting protein 1 like protein; RPGR-interacting protein 1-like protein; RPGRIP1 like protein; RPGRIP1-like protein; Rpgrip1l; anti-RPGRIP1L antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPGRIP1L antibody detects endogenous levels of RPGRIP1L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1235

• Applicable Applications for anti-RPGRIP1L antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human RPGRIP1L.
• Subcellular Location: Cytoplasm. Cytoplasm > Cytoskeleton > Cilium Basal Body. Cytoplasm > Cytoskeleton > Cilium Axoneme. Cytoplasm > Cytoskeleton > Microtubule Organizing Center > Centrosome. Cell Junction > Tight Junction. In cultured renal cells, it localizes diffusely in the cytoplasm but, as cells approach confluence, it accumulates to basolateral tight junctions.
• Tissue Specificity: Ubiquitously expressed with relatively high level of expression in hypothalamus and islet. During early development, expressed in multiple organs including brain, eye, forelimb and kidney.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (100%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603399 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of human kidney tissue, using RPGRIP1L antibody.)

Related Product Information for anti-RPGRIP1L antibody

Function: Negatively regulates signaling through the G-protein coupled thromboxane A2 receptor (TBXA2R) (PubMed:19464661). May be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis (By similarity). Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (PubMed:19464661). Involved in establishment of planar cell polarity such as in cochlear sensory epithelium and is proposed to implicate stabilization of disheveled proteins (By similarity). Involved in regulation of proteasomal activity at the primary cilium probably implicating association with PSDM2 (By similarity).
Subunit Structure: Interacts with NPHP4 and NPHP1; NPHP1, NPHP4 and RPGRIP1L are proposed to form a functional NPHP1-4-8 module localized to cell-cell contacts and the ciliary transition zone; NPHP4 mediates the interaction between NPHP1 and RPGRIP1L. Interacts with IQCB1; the interaction likely requires additional interactors (By similarity). Interacts with TBXA2R (via C-terminus). Interacts with RPGR. Interacts with NEK4. Interacts with NPHP4, INVS and DVL2; the complex is proposed to be involved in DVL2 stabilization.
Similarity: Belongs to the RPGRIP1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 189217904
• NCBI GeneID: 23322
• NCBI Accession #: NP_001121369.1
• NCBI GenBank Nucleotide #: NM_001127897.3
• UniProt Accession #: Q68CZ1; Q9Y2K8; A0PJ88
• Molecular Weight: Observed: 151 kDa; Predicted: 152 kDa
• NCBI Official Full Name: protein fantom isoform b
• NCBI Official Synonym Full Names: RPGRIP1 like
• NCBI Official Symbol: RPGRIP1L
• NCBI Official Synonym Symbols: FTM; MKS5; CORS3; JBTS7; NPHP8; PPP1R134
• NCBI Protein Information: protein fantom
• UniProt Protein Name: Protein fantom
• UniProt Gene Name: RPGRIP1L
• UniProt Synonym Gene Names: FTM; KIAA1005; NPHP8; RPGRIP1-like protein

NCBI Description

The protein encoded by this gene can localize to the basal body-centrosome complex or to primary cilia and centrosomes in ciliated cells. The encoded protein has been found to interact with nephrocystin-4. Defects in this gene are a cause of Joubert syndrome type 7 (JBTS7) and Meckel syndrome type 5 (MKS5). [provided by RefSeq, Jun 2016]

Uniprot Description

Negatively regulates signaling through the G-protein coupled thromboxane A2 receptor (TBXA2R) (PubMed:19464661). May be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis (). Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (PubMed:19464661). Involved in establishment of planar cell polarity such as in cochlear sensory epithelium and is proposed to implicate stabilization of disheveled proteins (). Involved in regulation of proteasomal activity at the primary cilium probably implicating association with PSDM2 ().