Rabbit RPA32 Polyclonal Antibody | anti-RPA2 antibody

RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34)

Cat. Num. AAA626778

• Gene Names: RPA2; REPA2; RPA32
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RPA32; Polyclonal Antibody; RPA32 (Replication Protein A 32kD Subunit; Replication Factor A Protein 2; Replication Protein A2 (32kD); Replication Protein A2 32kDa; REPA2; RPA2; HSSB; p32; p34; RF-A Protein 2; RPA p32; RP-A p34; RPA34); Anti -RPA32 (Replication Protein A 32kD Subunit; anti-RPA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human p32. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-RPA2 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 5ug/ml; Immunocytochemistry: 1:500
• Immunogen: Cocktail of peptides corresponding to the N-terminus of human and mouse p32.
• Positive Control: A431 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(mmunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of A431and HeLa cells using Anti-p32 (Red). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)

Western Blot (WB)

(Western Blotting Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-p32 (0.5 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates p32 (~32 kDa).)

Related Product Information for anti-RPA2 antibody

The p32 protein is a doughnut-shaped trimer that is primarily localized in the mitochondria. It has also been reported to be present at the cell surface and in the nucleus. The role of p32 in mammalian cells is unclear. The yeast p32 homologue has been reported to regulate oxidative phosphorylation, and a link to autophagy has been proposed. Elevated expression of p32 in cancer has also been noted. Additionally, p32 is primarily localized in hypoxic/nutrient-deprived regions within tumors and that, in addition to tumor cells, a tumor-associated macrophage/myeloid cell subpopulation closely linked to tumor lymphatics expresses high levels of p32. The expression of p32 at the cell surface, where it can be targeted with p32-binding antibodies and peptides, adds a second level of tumor specificity to p32 expression. The unique expression pattern of p32 in tumor cells, tumor lymphatics, and tumor-associated macrophages/myeloid cells makes p32 a potential target for the identification and therapy of cancer.

Product Categories/Family for anti-RPA2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4506585
• NCBI GeneID: 6118
• NCBI Accession #: NP_002937.1
• NCBI GenBank Nucleotide #: NM_002946.3
• UniProt Accession #: P15927; Q52II0; Q5TEI9; Q5TEJ5; Q5TEJ0; Q5TEJ7
• Molecular Weight: 29,247 Da
• NCBI Official Full Name: replication protein A 32 kDa subunit
• NCBI Official Synonym Full Names: replication protein A2, 32kDa
• NCBI Official Symbol: RPA2
• NCBI Official Synonym Symbols: REPA2; RPA32
• NCBI Protein Information: replication protein A 32 kDa subunit; RP-A p32; RP-A p34; RF-A protein 2; OTTHUMP00000004008; OTTHUMP00000004009; OTTHUMP00000004010; OTTHUMP00000004011; replication factor A protein 2; replication protein A 34 kDa subunit
• UniProt Protein Name: Replication protein A 32 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA2
• UniProt Synonym Gene Names: REPA2; RPA32; RPA34
• UniProt Entry Name: RFA2_HUMAN

Uniprot Description

RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: nucleoplasm; PML body; chromosome, telomeric region; DNA replication factor A complex; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; damaged DNA binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding

Biological Process: G1 DNA damage checkpoint; mismatch repair; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle