Rabbit anti-Human RORgamma Polyclonal Antibody | anti-RORgamma antibody

RORgamma polyclonal antibody

Cat. Num. AAA3015835

• Gene Names: RORC; TOR; RORG; RZRG; NR1F3; RZR-GAMMA
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: RORgamma; Polyclonal Antibody; RORgamma polyclonal antibody; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; RORC; NR1F3; RORG; RZRG; anti-RORgamma antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: RORgamma polyclonal antibody detects endogenous levels of RORgamma protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-RORgamma antibody: Western Blot (WB)
• Application Notes: WB: 1:1000~1:2000
• Immunogen: Synthetic peptide, corresponding to Human RORgamma.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of ROR? polyclonal antibody at 1:1000 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug))

Related Product Information for anti-RORgamma antibody

The nuclear orphan receptors RORalpha and RORg are members of the nuclear hormone receptor superfamily. Members of this family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORg are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORg comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered "orphan receptors," due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. The gene encoding RORalpha maps to chromosome 15q22.2 and the gene encoding RORg maps to chromosome 1q21.3.

NCBI and Uniprot Product Information

• NCBI GI #: 48255918
• NCBI GeneID: 6097
• NCBI Accession #: NP_001001523.1
• NCBI GenBank Nucleotide #: NM_001001523.1
• UniProt Accession #: P51449; Q5SZR9; Q8N5V7; Q8NCY8
• Molecular Weight: 58,195 Da
• NCBI Official Full Name: nuclear receptor ROR-gamma isoform b
• NCBI Official Synonym Full Names: RAR-related orphan receptor C
• NCBI Official Symbol: RORC
• NCBI Official Synonym Symbols: TOR; RORG; RZRG; NR1F3; RZR-GAMMA
• NCBI Protein Information: nuclear receptor ROR-gamma; nuclear receptor RZR-gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; RAR-related orphan receptor C, isoform a; RAR-related orphan nuclear receptor variant 2; nuclear receptor subfamily 1 gro
• UniProt Protein Name: Nuclear receptor ROR-gamma
• UniProt Gene Name: RORC
• UniProt Synonym Gene Names: NR1F3; RORG; RZRG
• UniProt Entry Name: RORG_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RORC: Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T-helper cells into Th17 cells. Regulate the expression of several components of the circadian clock. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; oxysterol binding; transcription factor activity

Biological Process: Peyer's patch development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; T-helper cell differentiation; regulation of fat cell differentiation; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; positive regulation of circadian rhythm; lymph node development; protein amino acid phosphorylation; regulation of gamma-delta T cell differentiation; xenobiotic metabolic process; regulation of steroid metabolic process; steroid hormone mediated signaling; gene expression; circadian regulation of gene expression