Rabbit anti-Human Ron  Polyclonal Antibody | anti-Ron  antibody

Ron (Phospho-S1394) polyclonal antibody

Cat. Num. AAA3005026

• Gene Names: MST1R; RON; SEA; PTK8; CD136; NPCA3; CDw136
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ron ; Polyclonal Antibody; Ron (Phospho-S1394) polyclonal antibody; Macrophage-stimulating protein receptor; MSP receptor; CDw136; Protein-tyrosine kinase 8; p185-Ron; CD_antigen; CD136; MST1R; PTK8; RON; anti-Ron antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ron (phospho-S1394) polyclonal antibody detects endogenous levels of Ron protein only when phosphorylated at Ser1394.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1351

• Applicable Applications for anti-Ron antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Ron around the phosphorylation site of Serine 1394
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ron  (phospho-S1394)  polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-Ron  antibody

Ron is a member of the Met protooncogene family of receptor tyrosine kinases, which also includes Stk, c-Met, and c-Sea. The functional Ron is a heterodimer composed of a 40 kDa alpha chain and a 150 kDa beta chain. Ron is initially synthesized in the cells as a single-chain, pro-Ron precursor that is cleaved into the two active chains. The alpha chain is completely extracellular, whereas the beta chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements. Ron mediates multiple signaling cascades that involve cell motility, adhesion, proliferation, and apoptosis. The signaling pathways activated downstream of Ron include the ras/mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3 kinase (PI3K)/Akt, and focal adhesion kinase (FAK) pathways. Ron activation can also significantly increase c-Src activity, a signaling intermediate involved in cell cycle progression, motility, angiogenesis and survival. The function of Ron has been shown to be important for embryological development as well as implicated in the progression and metastasis of tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 349732251
• NCBI GeneID: 4486
• NCBI Accession #: NP_001231866.1
• NCBI GenBank Nucleotide #: NP_001231866.1
• UniProt Accession #: Q04912
• Molecular Weight: ~ 145, 150kDa
• NCBI Official Full Name: macrophage-stimulating protein receptor isoform 2
• NCBI Official Synonym Full Names: macrophage stimulating 1 receptor
• NCBI Official Symbol: MST1R
• NCBI Official Synonym Symbols: RON; SEA; PTK8; CD136; NPCA3; CDw136
• NCBI Protein Information: macrophage-stimulating protein receptor
• UniProt Protein Name: Macrophage-stimulating protein receptor
• UniProt Gene Name: MST1R
• UniProt Synonym Gene Names: PTK8; RON; MSP receptor
• UniProt Entry Name: RON_HUMAN

NCBI Description

This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternative splicing generates multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

Uniprot Description

Ron: a receptor tyrosine kinase of the Met family. Receptor for macrophage stimulating protein. Can be desensitized by c-Cbl and its binding partner Grb2. Overexpression of wild-type RON causes the formation of lung tumors. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Met family

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cell surface; integral to plasma membrane; stress fiber

Molecular Function: macrophage colony stimulating factor receptor activity; protein binding; enzyme binding; ATP binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein kinase B signaling cascade; peptidyl-tyrosine phosphorylation; multicellular organismal development; response to virus; single fertilization; positive regulation of cell proliferation; innate immune response; defense response; signal transduction; cell motility; transmembrane receptor protein tyrosine kinase signaling pathway