Rabbit anti-Human, Mouse MYH7-specific Polyclonal Antibody | anti-MYH7 antibody

MYH7-specific Antibody

Cat. Num. AAA9603350

• Gene Names: MYH7; CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: MYH7-specific; Polyclonal Antibody; MYH7-specific Antibody; Beta myosin heavy chain; cardiac muscle beta isoform; CMD1S; CMH1; MPD1; MYH7; MYH7_HUMAN; Myhc slow; MyHC-beta; MyHC-slow; MYHCB; Myopathy; distal 1; Myosin heavy chain (AA 1-96); Myosin heavy chain 7; Myosin heavy chain; Myosin heavy chain slow isoform; Myosin; heavy chain 7; cardiac muscle; beta; heavy polypeptide 7; Myosin-7; Rhabdomyosarcoma antigen MU RMS 40.7A; SPMD; SPMM; anti-MYH7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYH7-specific antibody detects endogenous levels of MYH7-specific.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1935

• Applicable Applications for anti-MYH7 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human MYH7-specific.
• Subcellular Location: Cytoplasm > Myofibril. Thick filaments of the myofibrils.
• Tissue Specificity: Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Dog
• Similarity: Pig (92%), Bovine (92%), Horse (100%), Sheep (92%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603350 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of mouse heart tissue, using MYH7-specific antibody.)

Related Product Information for anti-MYH7 antibody

Function: Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
Subunit Structure: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with ECPAS (PubMed:20682791). Interacts (via C-terminus) with LRRC39 (PubMed:20847312).
Similarity: The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils (PubMed:26150528, PubMed:26573747). Four skip residues (Skip1: Thr-1188, Skip2: Glu-1385, Skip3: Glu-1582 and Skip4: Gly-1807) introduce discontinuities in the coiled-coil heptad repeats. The first three skip residues are structurally comparable and induce a unique local relaxation of the coiled-coil superhelical pitch and the fourth skip residue lies within a highly flexible molecular hinge that is necessary for myosin incorporation in the bare zone of sarcomeres (PubMed:26150528).Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

NCBI and Uniprot Product Information

• NCBI GI #: 115496169
• NCBI GeneID: 4625
• NCBI Accession #: NP_000248.2
• NCBI GenBank Nucleotide #: NM_000257.3
• UniProt Accession #: P12883; Q14836; Q14837; Q14904; Q16579; Q2M1Y6; Q92679; Q9H1D5; Q9UDA2; Q9UMM8; A2TDB6; B6D424
• Molecular Weight: Observed: 200-220 kDa; Predicted: 224 kDa
• NCBI Official Full Name: myosin-7
• NCBI Official Synonym Full Names: myosin heavy chain 7
• NCBI Official Symbol: MYH7
• NCBI Official Synonym Symbols: CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB
• NCBI Protein Information: myosin-7
• UniProt Protein Name: Myosin-7
• Protein Family: Myosin
• UniProt Gene Name: MYH7
• UniProt Synonym Gene Names: MYHCB; MyHC-slow; MyHC-beta

NCBI Description

Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.