Rabbit anti-Human, Rat Muscarinic Receptor M1, aa227-353 Polyclonal Antibody | anti-CHRM1 antibody

Muscarinic Receptor M1, aa227-353 (Cholinergic Muscarinic Receptor M1, Chrm1, Muscarinic Receptor M1)

Cat. Num. AAA6007258

• Gene Names: CHRM1; M1; HM1; M1R
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Muscarinic Receptor M1; aa227-353; Polyclonal Antibody; aa227-353 (Cholinergic Muscarinic Receptor M1; Chrm1; Muscarinic Receptor M1); Anti -Muscarinic Receptor M1; anti-CHRM1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Muscarinic Receptor M1. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile, distilled, deionized water or sterile 40-50% glycerol.

• Applicable Applications for anti-CHRM1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Westen Blot.; Dilution: Western Blot: 1:200 Rat brain membranes
• Immunogen: Fusion protein, GSETP GKGGGSSSSS ERSQP GAEGS PETPP GRCCR CCRAP RLLQAYSWKE EEEED EGSME SLTSS EGEEP GSEVV IKMPM VDPEA QAPTK QPPRS SPNTV KRPTK KGRDR AGKGQ KPRGK EQLAK RK, corresponding to aa227-353 of human M1 (Accession P11229). Epitope location: 3rd intracellular loop.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blotting of total rat brain membrane proteins (50 mg/lane) 1. MBS6007258: 1:200 2. MBS6007258 preincubated with control fusion protein.)

Testing Data

(MBS6007258 staining of rat cortex neurons.)

Product Categories/Family for anti-CHRM1 antibody

Antibodies; Abs to Receptors; Cholinergic

NCBI and Uniprot Product Information

• NCBI GI #: 37622910
• NCBI GeneID: 1128
• NCBI Accession #: NP_000729.2
• NCBI GenBank Nucleotide #: NM_000738.2
• UniProt Accession #: P11229
• Molecular Weight: 51,421 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M1
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 1
• NCBI Official Symbol: CHRM1
• NCBI Official Synonym Symbols: M1; HM1; M1R
• NCBI Protein Information: muscarinic acetylcholine receptor M1; acetylcholine receptor, muscarinic 1
• UniProt Protein Name: Muscarinic acetylcholine receptor M1
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM1
• UniProt Entry Name: ACM1_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: asymmetric synapse; postsynaptic membrane; membrane; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; nerve terminal; cell junction

Molecular Function: drug binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: nervous system development; acetylcholine receptor signaling, muscarinic pathway; protein modification process; signal transduction; muscarinic acetylcholine receptor, phospholipase C activating pathway; regulation of locomotion; G-protein coupled receptor protein signaling pathway; cell proliferation; positive regulation of cell proliferation; protein kinase C activation; neuromuscular synaptic transmission; positive regulation of ion transport; cognition; saliva secretion