Rabbit anti-Human RNH1 Polyclonal Antibody | anti-RNH1 antibody

RNH1, CT (RNH1, PRI, RNH, Ribonuclease inhibitor, Placental ribonuclease inhibitor, Ribonuclease/angiogenin inhibitor 1) (FITC)

Cat. Num. AAA6343009

• Gene Names: RNH1; RAI; RNH
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNH1; Polyclonal Antibody; CT (RNH1; PRI; RNH; Ribonuclease inhibitor; Placental ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1) (FITC); Ribonuclease/angiogenin inhibitor 1; anti-RNH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 461

• Applicable Applications for anti-RNH1 antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RNH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-454 amino acids from the C-terminal region of human RNH1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNH1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the RNH1 antibody detected the RNH1 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of RNH1 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-RNH1 antibody

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Product Categories/Family for anti-RNH1 antibody

Antibodies; Enzymes; Ribonuclease

References

Martins-de-Souza, D., et al. J Psychiatr Res 44(14):989-991(2010). Martins-de-Souza, D., et al. J Neural Transm 116(3):275-289(2009). Turcotte, R.F., et al. Biochem. Biophys. Res. Commun. 377(2):512-514(2008). Johnson, R.J., et al. Biochemistry 46(45):13131-13140(2007). Johnson, R.J., et al. J. Mol. Biol. 368(2):434-449(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 42794608
• NCBI GeneID: 6050
• NCBI Accession #: NP_976318.1; NP_976317.1
• NCBI GenBank Nucleotide #: NP_976318.1
• UniProt Accession #: P13489
• NCBI Official Full Name: ribonuclease inhibitor
• NCBI Official Synonym Full Names: ribonuclease/angiogenin inhibitor 1
• NCBI Official Symbol: RNH1
• NCBI Official Synonym Symbols: RAI; RNH
• NCBI Protein Information: ribonuclease inhibitor
• UniProt Protein Name: Ribonuclease inhibitor
• Protein Family: Ribonuclease inhibitor
• UniProt Gene Name: RNH1
• UniProt Synonym Gene Names: PRI; RNH; Placental RNase inhibitor; RAI
• UniProt Entry Name: RINI_HUMAN

NCBI Description

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]

Uniprot Description

RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: angiogenin-PRI complex; cytoplasm

Molecular Function: protein binding; ribonuclease inhibitor activity

Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis